USTC/Notebook/Preparing Antibiotic Stocks

From 2008.igem.org

(Difference between revisions)
 
Line 8: Line 8:
Stocks & Usage
Stocks & Usage
-
Stock Concentration - 100mg/ml in H2O
+
*'''Stock Concentration''' - 50mg/ml in H<sub>2</sub>O
-
Aliquots - 200μl and 1ml
+
*'''Aliquots''' - 100&mu;l and 500&mu;l
-
Working Concentration - 100μg/ml  
+
*'''Working Concentration''' - 50&mu;g/ml
-
 
+
===Kanamycin===
===Kanamycin===
Stocks & Usage
Stocks & Usage
-
Stock Concentration - 10mg/ml in H2O
+
*'''Stock Concentration''' - 10mg/ml in H<sub>2</sub>O
-
Aliquots - 200μl and 1ml  
+
*'''Aliquots''' - 200μl and 1ml  
-
Working Concentration - 20μg/ml  
+
*'''Working Concentration''' - 20μg/ml  
===Chloramphenicol===
===Chloramphenicol===
-
Stock concentration - 34mg/ml in 100% Ethanol  
+
Stocks & Usage
-
Aliquots - 1ml  
+
*'''Stock concentration''' - 34mg/ml in 100% Ethanol  
-
Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed)
+
*'''Aliquots''' - 1ml  
 +
*'''Working concentration''' = 25μ/ml (Stringent), 170μ/ml (relaxed)

Latest revision as of 13:58, 27 October 2008

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Contents

Preparing Antibiotic Stocks

Ampicillin

Stocks & Usage

  • Stock Concentration - 50mg/ml in H2O
  • Aliquots - 100μl and 500μl
  • Working Concentration - 50μg/ml

Kanamycin

Stocks & Usage

  • Stock Concentration - 10mg/ml in H2O
  • Aliquots - 200μl and 1ml
  • Working Concentration - 20μg/ml

Chloramphenicol

Stocks & Usage

  • Stock concentration - 34mg/ml in 100% Ethanol
  • Aliquots - 1ml
  • Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed)
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