Wisconsin:Lignin Project/28 August 2008

From 2008.igem.org

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srlD PCR product was digested with biobrick vector pSB1AK3 recieved from the registry.<br>
srlD PCR product was digested with biobrick vector pSB1AK3 recieved from the registry.<br>
Digestion was verified on a gel and purified through gel extraction. <br>
Digestion was verified on a gel and purified through gel extraction. <br>
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Overnight ligation of insert into pSB1AK3 was allowed to run overnight.
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Overnight ligation of insert into pSB1AK3 was allowed to run overnight.<br>
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'''Team Lignin:'''<br>
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Ran Azure B assay on 96 well plate.<br>
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Revision as of 02:42, 29 October 2008

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Team Sorbitol:

New primers arrived for gap between srlA, and srlE.
Performed new sequencing PCR reaction.
srlD PCR product was digested with biobrick vector pSB1AK3 recieved from the registry.
Digestion was verified on a gel and purified through gel extraction.
Overnight ligation of insert into pSB1AK3 was allowed to run overnight.

Team Lignin:
Ran Azure B assay on 96 well plate.