Wisconsin:Lignin Project/29 August 2008

From 2008.igem.org

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{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
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|'''Team Sorbtitol:'''<br>
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|'''Team Sorbitol:'''<br>
Performed PCR cleanup and reactions for sequencing.<br>
Performed PCR cleanup and reactions for sequencing.<br>
Submitted for sequencing.<br>
Submitted for sequencing.<br>
Sequencing results received same day. All colonies perfect matches with new primers. Success!<br>
Sequencing results received same day. All colonies perfect matches with new primers. Success!<br>
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Ligation product transformed into E.coli.
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Ligation product transformed into E.coli.<br>
 +
Second His-tag purification resulted again with no protein.  Now it is believed to be too small an amount for the nanodrop to measure.
 +
 
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Revision as of 00:21, 29 October 2008

Igemwibanner.gif
Team Sorbitol:

Performed PCR cleanup and reactions for sequencing.
Submitted for sequencing.
Sequencing results received same day. All colonies perfect matches with new primers. Success!
Ligation product transformed into E.coli.
Second His-tag purification resulted again with no protein. Now it is believed to be too small an amount for the nanodrop to measure.