Wisconsin: Lignin Project/11 July 2008
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(New page: == Team Sorbitol == Froze down 3 400ul samples of the overnight DH5a-pBAD30-Srld E.coli for future use. Harvested the DNA from the pBAD30, PBAD30-srlD, and pBAD18 using a miniprep kit. ...) |
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- | + | <div style="width:800px; height:147px; border:0px; margin:0px;">[[Image:Igemwibanner.gif]]</div> | |
- | Harvested the DNA from the pBAD30, PBAD30-srlD, and pBAD18 using a miniprep kit. | + | {|align="justify" style="color:#aada84;background-color:#000;" width="800 px" |
- | + | |'''Team Sorbitol'''<br> | |
- | created chemically competent stocks of MG1655, JW3890 and RL357. | + | Froze down 3 400ul samples of the overnight DH5a-pBAD30-Srld E.coli for future use.<br> |
- | + | Harvested the DNA from the pBAD30, PBAD30-srlD, and pBAD18 using a miniprep kit.<br> | |
- | The stocks were frozen down and kept at -80C for future use. | + | created chemically competent stocks of MG1655, JW3890 and RL357.<br> |
+ | The stocks were frozen down and kept at -80C for future use.<br> | ||
+ | changing growth temperature increased growth of tpiA knockout greatly.<br> | ||
+ | '''Team Fungus'''<br> | ||
+ | Researched the necessary environment for the disulfide bond formation needed in correct folding of LiP <br> | ||
+ | Concluded that a oxidative cytoplasm was needed, thus the Novagen Origami strains were chosen as final transformation hosts, specifically Rosetta-gami2(DE3).<br> | ||
+ | Ran out gels of all tubes in freezer. Found five tubes with correct size bands.<br> | ||
+ | |} |
Latest revision as of 03:30, 29 October 2008
Team Sorbitol Froze down 3 400ul samples of the overnight DH5a-pBAD30-Srld E.coli for future use. |