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Wisconsin: Lignin Project/19 June 2008
From 2008.igem.org
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| + | |'''Team Sorbitol:''' | ||
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| + | Purified the PCR product from yesterday - not enough DNA for a digestion<br> | ||
| + | Re-ran the PCR reaction from yesterday to get more product - however the reaction failed (no bands)<br> | ||
| + | In the meantime designed a digestion reaction to use once we get enough product.<br> | ||
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'''Team Fungus:''' <br> | '''Team Fungus:''' <br> | ||
Took spec reading of Carbon and Nitrogen-limited cDNA<br> | Took spec reading of Carbon and Nitrogen-limited cDNA<br> | ||
Worked on Wiki site<br> | Worked on Wiki site<br> | ||
Worked on sponsorship<br> | Worked on sponsorship<br> | ||
| - | Made overnight cultures of: | + | Made overnight cultures of: BL21 (x2), pBad33, pMcSG7, pet28a, pet28b, DH1 (x2), pBad21<br> |
| - | BL21 (x2) | + | |
| - | pBad33 | + | |
| - | pMcSG7 | + | |
| - | pet28a | + | |
| - | pet28b | + | |
| - | DH1 (x2) | + | |
| - | pBad21 | + | |
| - | <br> | + | |
Made and autoclaved more LB solution for future use <br> | Made and autoclaved more LB solution for future use <br> | ||
Designed new and longer primers <br> | Designed new and longer primers <br> | ||
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Revision as of 15:19, 8 August 2008
| Team Sorbitol:
Purified the PCR product from yesterday - not enough DNA for a digestion Team Fungus: |
