Edinburgh/1 September 2008

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Week 12

Monday 1 September 08

  • Minipreps M182 to M193:
    • M182 to M187: pSB1A2+cenA.
    • M188 to M193: crtBI+appY.
    • Followed by double digets using EcoRI and PstI (YAN)
  • Fusion PCR:
    • P91: P88 (SU1) and SOB2 ligation products using Forward primer zm1 and Reverse primer sob r1.
    • P92: P88 (SU1) and SOB2 ligation products using Forward primer zm1 and Reverse primer sob.rbs.
    • P93: P89 (ISO2) and SOB2 ligation products using Forward primer zm2 and Reverse primer sob r1.
    • P94: P89 (ISO2) and SOB2 ligation products using Forward primer zm2 and Reverse primer sob.rbs. (YAN)
  • Overnight cultures 1 to 4 of appY+dxs+lims from plate 155 (YAN)
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