From 2008.igem.org
Agenda
- Exchange Lab Techniques & Protocols
- plasmid prep using low media volume, big flask (Margaret)
- SAP treatment of vector to prevent self-ligation, success? (Grace/Krystle)
- Gel cutting using SYBRSafe+Blue Light, Yield Loss at each step(NW)
- 70min PNK+Oligo Annealing Protocol (NW)
- Updates of progress from the past two weeks
- Grace
- Re-replacement of BB-pRL1383a MCS
- Krystle
- GFPf w/ tt? GFP w/ tt?
- Margaret
- Rep, OriT, OriV, etc...
- aadA, Omega
- P1 Lytic
- Norman
- Bioplastic synthase operon (phaABCE) construction progress
- site directed mutagenesis of two genes phaE and "sigma master switch"
- Logistics
- Travel itinerary (NW)
- Sign Waiver (All)
Minutes
Present: <Person A>, <Person B>
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Action Items
Coming Up