"
Team:Hawaii/Notebook/2008-07-18
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
PCR of pRL1383a Parts
- Margaret
- for details concerning the running conditions, please look here.
- Ran a 0.8%gel at 95V.
Please refer to the annotated picture.
Extraction of Biobricks and Transformation in DB3.1
- Margaret
- streaked plates on Amp100 plates
- for details please look here.
Sequencing
- Grace
- Redid PCR reactions for GFP fusion, BB-pRL1383a, B0015, J04430, R0010
- Gel OK this time (95V for 50 min; ladder resolved, loading dye didn't run funny)
- Incorrect bands still for B0015, J04430, R0010; Krystle will redo the plasmid preps for these
- Correct bands for GFP fusion and BB-pRL1383a. BB-pRL1383a band faint -- why?
- Treated PCR products w/ ExoSAP
- Determined DNA concentrations using nanodrop spectrometer (measured twice)
| PCR Sample | Concentration (1st measurement) | Concentration (2nd measurement) |
|---|---|---|
| nir | 1015 ng/μl | 1010 ng/μl |
| slr2016-1 | 1295 ng/μl | 1107 ng/μl |
| slr2016-2 | 1518 ng/μl | 1266 ng/μl |
| pilA | 1312 ng/μl | 1330 ng/μl |
| B0024 | 1546 ng/μl | 1474 ng/μl |
| B0034 | 1537 ng/μl | 1386 ng/μl |
| C0012 | 2130 ng/μl | 1530 ng/μl |
| E0040 | 1502 ng/μl | 1236 ng/μl |
| J33207 | 1454 ng/μl | 1223 ng/μl |
| BB-pRL1383a | 2279 ng/μl | 2129 ng/μl |
| GFP fusion | 1660 ng/μl | 1714 ng/μl |
- Sent 22 samples to CORE Hawaii for sequencing
Media Making
Margaret, Krystle (thanks for the help!)
- Made one sleeve of Amp100 plates.
Plasmid Prep of GFP fusion Brick, R0010, J3340
- Krystle
- did a large scale (75 ml) plasmid prep for the GFP fusion brick
- Performed 3 mini preps each of the BioBrick parts
Drylab Work
Project Description (Abstract)
- Grace, Krystle, Margaret
- Wrote description of project (abstract)
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
