"
Team:Hawaii/Notebook/2008-08-26
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Ran RE digests on gel
- Grace
- Gel did not resolve. Bands were convex, poor separation between bands. Bad buffer?
- Redid RE digests from yesterday
- Used PCR products of nir and J33207
Inoculated for plasmid prep
- Grace
- BB-pRL1383a and J33207 in TB+amp100
Gel Purified
- Krystle
- Ran overnight ligation products on a 3% agarose gel
- discussion: If we decide to do the 3A in two parts, we will have to re-restriction digest the first part before moving on
Transformation of ligated GFPf + TT
- Krystle
- Performed transformation protocol with DB3.1 and GFPf + tt ligation from 08/25
Ligation
Margaret
- Overnight in 4°C
- rep+pSB1A3 (1:1)
- rep+pSB1A3 (1:3)
- rep+pSB1A3 (1:6)
- P1 lytic +pSB1A3 (1:1)
- P1 lytic +pSB1A3 (1:3)
- P1 lytic +pSB1A3 (1:6)
- [Plac B0030]+pSB1A3 (1:3)
- [Plac B0030]+pSB1A3 (1:6)
- [Plac B0034]+pSB1A3 (1:6)**ran out of vector
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
