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Team:Hawaii/Notebook/2008-09-10
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of p+r + s(+gf)
- Grace
- Ran last night's RE digest on gel
- Could not resolve nir+rbs, plac+rbs, slr1, pilA (too little DNA? flanking bands also close to size of desired band)
- Cut out slr1+GFPf, pilA+GFPf bands
- Treated RE digested pSB1A3 with SAP
- RE digest overnight:
- EcoRI + SpeI:
- nir+rbs (PCR product)
- SpeI + PstI:
- nir+rbs (plasmid prep)
- plac+rbs (plasmid prep)
- XbaI + PstI:
- slr1 (PCR product)
- pilA (PCR product)
Preparation for sequencing
- Grace
- plac + rbs colonies 1, 2, 10, 12 (#10 is potential successful transformant)
- C0012 derived vector used in plac+rbs 3A ligation, dephosphorylated colony 2 and phosphorylated colony 1
- slr1 + GFPf
- pilA + GFPf, 8/12 and 8/13 transformations
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
