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Things we did today

Wetlab work

Plasmid prep

Grace

• 5 ml plasmid prep of:

• BB-pRL1383a
• 3A: nir+rbs+slr1 #2, 3
• 3A: nir+rbs+pilA #6
• 3A: nir+rbs+slr1+GFPf #10
• 3A: nir+rbs+pilA+GFPf12 # 2, 6
• 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10

Gel Purification

Krystle

• gel purified restriction digest products of:
  • nir
  • pilA
  • slr
  • pSB1A3
  • GFPf + tt #8
  • GFPf + tt #17
  • GFPf + tt #21

Gel Verify constructs

Gel used to verify the insertion of Promoter and RBS in front of aada(prl&bb).

Margaret

• verified constructs: ap36, ab92, ab94, ab95, ab96, ab98
• verified constructs will be gel purified and used in a ligation.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

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