Edinburgh/18 September 2008

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== Week 14 ==
== Week 14 ==

Revision as of 18:19, 6 October 2008

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Week 14

Thursday 18 September ~ Friday 19 September 08

  • Sent M213, M218 (dxs+crtEBI) for sequencing. (AM)
  • Single (EcoRI) and double (EcoRI+PstI) digests of:
    • M198 (SOB2+zm1)
    • M201~203 (SOB2-rbs+zm1)
    • M204~206 (SOB2+zm2)
    • M207~209 (SOB2-rbs+zm2). (Yan)
  • Single (PstI) digest of P97 (cex mut). (AM)
  • M198~M206 run on Gel 80. All failed: single and double digests yielded the same results for all samples. (Yan)
  • M207~209 and P97 run on Gel 81. All failed: single and double digests yielded the same results for M207~209. PstI digest of P97 resulted in a smear. (AM)
  • Transformation/plating of CHU1727 failed twice. (CKL)