Team:Hawaii/Notebook/2008-10- 7

From 2008.igem.org

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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of secretion device (cont.)=== :<strong> Grace</strong> :* Ran RE digests on gel :* Extracted bands from ...)
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==Drylab Work==
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:<strong>Grace, Krystle, Margaret</strong>
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:* Revised project abstract
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= Discussion =

Revision as of 02:04, 9 October 2008

Projects Events Resources
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Notebook (t) Meetings (t)

Things we did today

Wetlab work

Construction of secretion device (cont.)

Grace
  • Ran RE digests on gel
  • Extracted bands from gel
  • Ligated:
  • nir + rbs+gfp+tt#1
  • plac + rbs+gfp+tt#1
  • nir+rbs + slr1+GFPf
  • plac+rbs + slr1+GFPf
  • nir+rbs + pilA+GFPf+tt
  • plac+rbs + pilA+GFPf+tt
  • pSB1A3 to self (control)
  • Used 5 μl ligation mixture to transform DH5α cells


Drylab Work

Grace, Krystle, Margaret
  • Revised project abstract

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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