Team:Paris/September 1

From 2008.igem.org

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(Construction of pFlhB - mRFP Tripart (LVA+))
 
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=Construction of pFlhB - mRFP Tripart (LVA+)=
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=Construction of rbs-FlhDC-mRFP-LVA-tripart (L172)=
Aim : Construction of ''' "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078)''' [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
Aim : Construction of ''' "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078)''' [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
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<br>We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.
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<br>==> it appears a mistake of name digestion, it explains the realisation of a construction not wanted
==Digestion==
==Digestion==
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|16
|16
|1.31
|1.31
 +
|-
 +
|D186
 +
|24
 +
|1.25
|}
|}
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|L168
|L168
|D187
|D187
-
|
+
|2.5
|D186
|D186
-
|
+
|1
|-
|-
|Control L168
|Control L168
|D166
|D166
-
|
+
|2.5
| -
| -
|}
|}

Latest revision as of 14:49, 13 September 2008

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Contents

Cloning of EnvZ*

Transformation results of L165 (EnvZ*-pSB1A2) from August 29th

sample number of colonies
positive control pUC19 many colonies
transformation negative control 0
ligation without insert 0
3:1 insert:vector ratio 5
4:1 insert / vector ratio 5


Construction of rbs-FlhDC-mRFP-LVA-tripart (L172)

Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) Part icon regulatory.pngPart icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
==> it appears a mistake of name digestion, it explains the realisation of a construction not wanted

Digestion

Measurement of the concentration of D187 purified

Protocol (it's same that for Miniprep)

Digestion Name Concentration (µg/mL) Ratio 260/280
D187 16 1.31
D186 24 1.25

Ligation

Protocol

Ligation Name Vector Name Volume Vector (µL) Insert Volume Insert (µL)
L168 D187 2.5 D186 1
Control L168 D166 2.5 -