Team:Paris/July 17

From 2008.igem.org

(Difference between revisions)
(→Results of the transformations (Biobricks 2007))
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{{Paris/Calendar_Links|July 16|July 18}}
{{Paris/Calendar_Links|July 16|July 18}}
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|align="center"|a lot
|align="center"|a lot
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==Cloning of the transformations==
==Cloning of the transformations==
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* Test 8 clones by Biobricks
* Test 8 clones by Biobricks
* Incubate O/N at 37°C on proper plates
* Incubate O/N at 37°C on proper plates
 +
 +
==Screening PCR of the transformations==
==Screening PCR of the transformations==
 +
Use of the same 8 clones of Biobricks that have been tested for cloning
 +
 +
 +
===Mix for PCR (x76 tubes)===
-
====Mix for PCR (x76 tubes)====
 
25µl Quick load 2x
25µl Quick load 2x
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* 50µl of Mix PCR by tubes
* 50µl of Mix PCR by tubes
* 1 clone
* 1 clone
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* Programme : 105°C -  for 28 cycles : 95°C; 5' - 95°C; 30" - 55°C; 30" - 72°C; 1,5'
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* Programme : 105°C -  for 29 cycles : 95°C; 5' - 95°C; 30" - 55°C; 30" - 72°C; 1,5'

Revision as of 19:14, 17 July 2008

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Contents

Results of the transformations (Biobricks 2007)

Legende :

  • few , medium, a lot --> description of the number of CFU (Colony forming unit) produce by the transformation
  • A: Ampicilin, K: Kanamycin


Ref. Book Biobricks 2007 Antibio Results
- positive control A 2.800 CFU
- negative control A 0 CFU
2E_SP 1000 B0034 A few
10F_SP 1001 R0079 A medium
4F_SP 1013 C0079 K few
6H_SP 1001 C0179 A medium
6B_SP 1001 S03154 A 0 CFU
10F_SP 1002 J23101 A a lot
11F_SP 1002 J23109 A a lot
4C_SP 1000 R0040 A 2 CFU
2G_SP 1000 C0040 A 0 CFU
1C_SP 1008 PSB1A3 A few
7G_SP 1013 E1010 K medium
7E_SP 1001 E0040 A 2 CFU
2F_SP 1016 E0030 K a lot


Cloning of the transformations

  • Test 8 clones by Biobricks
  • Incubate O/N at 37°C on proper plates


Screening PCR of the transformations

Use of the same 8 clones of Biobricks that have been tested for cloning


Mix for PCR (x76 tubes)

25µl Quick load 2x 1µl Oligo F 1µl Oligo R 23µl H2O


Protocol

  • 50µl of Mix PCR by tubes
  • 1 clone
  • Programme : 105°C - for 29 cycles : 95°C; 5' - 95°C; 30" - 55°C; 30" - 72°C; 1,5'
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