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Team:The University of Alberta/21 July 2008
From 2008.igem.org
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**Results in. Didn't work. Again. Either we're doing the Westerns incorrectly or the butanol parts aren't being translated for some reason. | **Results in. Didn't work. Again. Either we're doing the Westerns incorrectly or the butanol parts aren't being translated for some reason. | ||
*Worked out the procedure for putting together all of our parts. | *Worked out the procedure for putting together all of our parts. | ||
| + | *Re-digesting Lac1 ERE, TetR. RBS.6x His, RBS-6xHis, TDNA-MCS with Xba/Pst. | ||
Revision as of 20:40, 21 July 2008
Today
- Transformed the ligation of Tryp into J6
- Finished Westerns of the Butanol suff in J6...RESULTS PENDING
- Results in. Didn't work. Again. Either we're doing the Westerns incorrectly or the butanol parts aren't being translated for some reason.
- Worked out the procedure for putting together all of our parts.
- Re-digesting Lac1 ERE, TetR. RBS.6x His, RBS-6xHis, TDNA-MCS with Xba/Pst.
