Team:Paris/August 13
From 2008.igem.org
(Difference between revisions)
(→PCR screening) |
(→PCR screening) |
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*10 µL loaded | *10 µL loaded | ||
[[Image:Screening-PCR-080813.JPG|thumb|'''Gel 1''': PCR screening ('''2-7''') & PCR product purification using the Qiaquick Gel Extraction kit ('''8''')]] | [[Image:Screening-PCR-080813.JPG|thumb|'''Gel 1''': PCR screening ('''2-7''') & PCR product purification using the Qiaquick Gel Extraction kit ('''8''')]] | ||
+ | |||
+ | {|border="1" | ||
+ | |align=center|'''well n°''' | ||
+ | |align=center|1 | ||
+ | |align=center|2 | ||
+ | |align=center|3 | ||
+ | |align=center|4 | ||
+ | |align=center|5 | ||
+ | |align=center|6 | ||
+ | |align=center|7 | ||
+ | |align=center|8 | ||
+ | |- | ||
+ | |align=center|'''sample''' | ||
+ | |align=center|100 bp DNA ladder | ||
+ | |align=center|positive control | ||
+ | |align=center|negative control | ||
+ | |align=center|L130.3 | ||
+ | |align=center|L130.4 | ||
+ | |align=center|L130.5 | ||
+ | |align=center|L130.6 | ||
+ | |align=center|PCR product (L130.8) purified by the Qiaquick Gel Extraction kit | ||
+ | |- | ||
+ | |align=center|'''red fluorescence''' | ||
+ | |align=center|not concerned | ||
+ | |align=center|strain a little bit pink | ||
+ | |align=center|not concerned | ||
+ | |align=center|no | ||
+ | |align=center|no | ||
+ | |align=center|no | ||
+ | |align=center|no | ||
+ | |align=center|not concerned | ||
+ | |- | ||
+ | |align=center|'''expected size''' | ||
+ | |align=center| | ||
+ | |align=center|1,161 kb | ||
+ | |align=center|0 kb | ||
+ | |align=center|1,338 kb | ||
+ | |align=center|1,338 kb | ||
+ | |align=center|1,338 kb | ||
+ | |align=center|1,338 kb | ||
+ | |align=center|doesn't matter | ||
+ | |- | ||
+ | |align=center|'''measured size''' | ||
+ | |align=center| | ||
+ | |align=center| | ||
+ | |align=center| | ||
+ | |align=center| | ||
+ | |align=center| | ||
+ | |align=center| | ||
+ | |align=center| | ||
+ | |align=center| | ||
+ | |} | ||
==Promoter Characterization: work plan== | ==Promoter Characterization: work plan== |
Revision as of 12:20, 14 August 2008
Sequencing Minipreps we did yesterday
Minipreps: Plasmid extraction
Glycerol Stocks
PCR screening4 more transformants of L130 (pFlhB into J61002) are screened by PCR.
Electrophoresis
Promoter Characterization: work plan |