Team:Paris/August 25
From 2008.igem.org
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Revision as of 19:29, 26 August 2008
Construction for SynchronizationTransformation of the ligations we did yesterday
Construction of pFlgA - GFP GeneratorAim : Construction of "pFlgA-RBS-GFP-dbl ter" (pFlgA-E0240)
Results of the transformation we did the day before yesterday
=> Need to screen to know which clones we can use for the of pFlgA promotor characterization. Cloning of EnvZ*The sequencing of EnvZ* previously cloned, revealed a loss of about 300 bp. EnvZ* contains indeed an EcoRI restriction site within its sequence. So we can't use this enzyme during the cloning. Digestion
Reaction mixture
Incubation at 37°C during 2H25, and then ~20 min at 65°C Electrophoresis1% agarose gel
elution in 30 µL of buffer EB
Promoter characterization plasmidsTransformation results: ligations from August 21thTop 10 cells were used
Screening of the cloning of pFlgA-YFP Tripart (LVA+/-)Bold text==Electrophoresis==
Minipreps and glycerol stock
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