Team:The University of Alberta/3 July 2008

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(New page: ===Today=== '''Chris'''<br> *After staining the gels from yesterday, there were no apparent bands. Could the Ni-NTA column purifications not have worked? Will run two more gels, with the c...)
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Revision as of 16:58, 3 July 2008

Today

Chris

  • After staining the gels from yesterday, there were no apparent bands. Could the Ni-NTA column purifications not have worked? Will run two more gels, with the crude and uninduced purified samples as well as induced pure.

Jason

  • Gel purified PCR products from the colony PCR yesterday
  • Sequenced the purified products

Saima

  • Troubleshooting: tried to determine the source of the strange bands from yesterday's colony PCR (esp. the bands in the water controls)

Tom

  • Made two SDS-PAGE gels for Chris to use (see above)