Alberta NINT/18 July 2008

From 2008.igem.org

(Difference between revisions)
Sdeane (Talk | contribs)
(New page: < Back to notebook < Previous entry lab work (SD): The double-plasmid cell cultures showed one-two colonies ...)
Newer edit →

Revision as of 21:26, 18 July 2008

< Back to notebook

< Previous entry

lab work (SD):

            The double-plasmid cell cultures showed one-two colonies on the LB+amp+kan35 plates.
            To test transformation efficiency, we will plate 150ul of XL1-B with pSB4A5+pSB4K5 on LB+amp50, 
            LB+kan35, and LB+amp50+kan35 plates using different amounts of plasmid and cells.
                              pSB4A5 (ul)   pSB4K5 (ul)   XL1-B (ul)
                  A              1.0           1.0           100
                  B              4.0           4.0           100
                  C              1.0           1.0            50
                  D              2.0           2.0            50


Next entry >