Team:Paris/August 28

From 2008.igem.org

(Difference between revisions)
(→Cloning of EnvZ*)
(→Digestion)
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*pSB1A2: excision of the band of interest (2 kb) and then purification using the Qiagen kit
*pSB1A2: excision of the band of interest (2 kb) and then purification using the Qiagen kit
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='''Digestion'''=
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=Freezer empty=
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==Repeat of Digestion==
[[Team:Paris/Notebook/Protocols#Digestion |Protocol]]
[[Team:Paris/Notebook/Protocols#Digestion |Protocol]]
We did again [[Team:Paris/Notebook/Freezer#Digestion Products |D110]] and  [[Team:Paris/Notebook/Freezer#Digestion Products |D125]].
We did again [[Team:Paris/Notebook/Freezer#Digestion Products |D110]] and  [[Team:Paris/Notebook/Freezer#Digestion Products |D125]].

Revision as of 19:08, 9 September 2008

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Contents

Transformation of OmpR*

Cloning of EnvZ*

The cloning of EnvZ* didn't worked. So we decided to try one more time, starting from the PCR product.

Digestion

Digestion of EnvZ* and lasR-pSB1A2
Digestion n° Name volume of DNA digested total volume reaction enzymes used
D159 PCR147 (EnvZ*) 3 µL 30 µL XbaI & PstI
D116 MP108 (lasR-pSB1A2) 2 µL 30 µL XbaI & PstI


Purification

  • EnvZ*: directly by the Qiagen kit
  • pSB1A2: excision of the band of interest (2 kb) and then purification using the Qiagen kit

Freezer empty

Repeat of Digestion

Protocol

We did again D110 and D125.

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