Team:Paris/September 10

From 2008.igem.org

(Difference between revisions)
(→Checking our ligases (following))
(→Checking our ligases (following))
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|15
|15
|-
|-
-
|'''
+
|'''sample'''
 +
|rowspan="5"|1 kb <br> DNA ladder
 +
|rowspan="5"|undigested DNA <br> MP101.1
 +
|colspan="12"|ligation products
 +
|rowspan="5"|100 bp <br> DNA ladder
 +
|-
 +
|'''temperature of ligation'''
 +
|colspan="8"|16°C
 +
|colspan="4"|4°C
 +
|-
 +
|'''ligase n°'''
 +
|colspan="4"|L1
 +
|colspan="4"|L2
 +
|colspan="2"|L1
 +
|colspan="2"|L2
 +
|-
 +
|'''duration of ligation'''
 +
|colspan="2"|2 h
 +
|colspan="2"|O/N
 +
|colspan="2"|2 h
 +
|colspan="6"|O/N
 +
|-
 +
|'''amount of ligase (units)'''
 +
|400 U
 +
|2 U
 +
|400 U
 +
|2 U
 +
|400 U
 +
|2 U
 +
|400 U
 +
|2 U
 +
|400 U
 +
|2 U
 +
|400 U
 +
|2 U
 +
|-
 +
|}

Revision as of 11:18, 11 September 2008

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Checking our ligases (following)

Analysis of the ligation products by electrophoresis

KR000310.JPG
well n° 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
sample 1 kb
DNA ladder 		undigested DNA
MP101.1 		ligation products 100 bp
DNA ladder
temperature of ligation 16°C 4°C
ligase n° L1 L2 L1 L2
duration of ligation 2 h O/N 2 h O/N
amount of ligase (units) 400 U 2 U 400 U 2 U 400 U 2 U 400 U 2 U 400 U 2 U 400 U 2 U
Retrieved from "http://2008.igem.org/Team:Paris/September_10"