Team:Paris/September 4
From 2008.igem.org
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(→Construction of pFlhB-mRFP-LVA-tripart & pFliL-ECFP-LVA-tripart) |
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- | =>the measurement of the absorbance was so instable, that we have prefered to do it again but we an another thechnik : by analysis on gel. | + | =>the measurement of the absorbance was so instable, that we have prefered to do it again but we an another thechnik : by analysis on gel. |
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==Ligation== | ==Ligation== |
Revision as of 18:38, 16 September 2008
Construction of rbs-TetR-mRFP-LVA-tripart (L173)PCR Screening of L173 transformantsTransformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3)Ligation L173
PCR ScreeningPCR screening programm
Digestion
Construction of pFlhB-mRFP-LVA-tripart & pFliL-ECFP-LVA-tripart
Measurement of the concentration of D168 purifiedProtocol (it's same that for Miniprep)
=>the measurement of the absorbance was so instable, that we have prefered to do it again but we an another thechnik : by analysis on gel. Ligation
=>it has been missed to do adaptated control. |