Team:ESBS-Strasbourg/Protocols

From 2008.igem.org

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==E.Coli==
==E.Coli==
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*'''Preparation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Competent Bacteria | Competent Bacteria]] <br>
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*[[Team:ESBS-Strasbourg/Competent Bacteria | Preparation of competent cells (Barbara's Protocol)]] <br>
*'''Preparation of TOP10 chemically competent cells''' [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol] <br>
*'''Preparation of TOP10 chemically competent cells''' [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol] <br>

Revision as of 23:53, 28 October 2008


E.Coli

  • Preparation of TOP10 chemically competent cells [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol]
  • Transformation of competent cells (Mariels/Barbaras Protocol) Transformation


Cloning

Solutions and buffers

  • SOB for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB]
  • SOC for bacterial transformation purposes [http://openwetware.org/wiki/SOC SOC]
  • TENS for the do it yourself MiniPrep TENS



Further source for protocols: [http://www.protocol-online.org/ Protocol-online.org]


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