Parts submitted

Following you can find a summary of our contribution to the registry.

Toolbox for modular transcription factors

DNA binding domains

• tetR BBa_K105003
• cI BBa_K105004
• lexA BBa_K105005
• lacI BBa_K105006
• Gal4 BBa_K105007

Activation domain

• VP16 BBa_K105001

Repression domain

• TUP1 BBa_K105002

Fluorescent markers

• CFP BBa_K105009
• mCherry BBa_K105010
• mOrange BBa_K105011

Junctions

• linker BBa_K105012

Tags

• cin8 - cell cycle dependent degradation BBa_K105013
• hsl1 – cell cycle dependent degradation BBa_K105015

=> all these parts are in our new plasmid derivate pSB1A2_fusion

Toolbox for modular promoters

Minimal promoters of different strength

• cyc100 BBa_K105027
• cyc70 BBa_K105028
• cyc43 BBa_K105029
• cyc28 BBa_K105030
• cyc16 BBa_K105031

Operator sites

• tetR operator BBa_K105020
• cI operator BBa_K105021
• lexA operator BBa_K105022
• lacI operator BBa_K105023
• Gal4 operator BBa_K105024

Yeast selection markers

• Leu2 - autotrophy marker BBa_K105016
• Ura3 - autotrophy marker BBa_K105017

Remakes of good, but not available BioBricks

• Gal1 promoter BBa_K105026
• Adh1 terminator BBa_K105025