Team:ESBS-Strasbourg/Essential publications
From 2008.igem.org
Essential publications
Project essentials
- Ajo-Franklin et al. Rational design of memory in eukaryotic cells. Genes & Development (2007) vol. 21 (18) pp. 2271-6 PMID 17875664
Eukaryotic activation/repression domains
Prokaryotic DNA binding domains
Protein degradation motifs
Nuclear localistation
Site-specific proteases
mRNA instability
BioBricks assembly
- Ira E. Philips, Pam Silver. A New Biobrick Assembly Strategy Designed for Facile Protein Engineering [http://dspace.mit.edu/handle/1721.1/32535]
Toggle switches
Fluorescent proteins
- [http://www.nature.com/nmeth/journal/v2/n12/abs/nmeth819.html;jsessionid=C9797F1441125016036E43C03175DD0D Shaner C.,Nature Methods (2005) -"A guide to choosing fluorescent proteins"]
- Propose cyan,yellow,orange and red => minimal crosstalk
- [http://www.clontech.com/products/detail.asp?product_id=10426&product_group_id=1437&product_family_id=1417&tabno=2 Reef Coral Fluorescent Proteins -Clontech]
- Said to be suitable for multiple labeling but forming tetramers (toxicity)