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Team:Hawaii/Notebook/2008-08-31
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of p+r (cont.) and re-replacement of BB-pRL1833a MCS
- Grace
- Ran RE digests from last night on gel
- plac+rbs band not visible; will ligate using plac and rbs parts (instead of plac+rbs part)
- Extracted bands from gel
- Ligated:
- plac and rbs (B0034) and C0012 vector
- J33207 and BB-pRL1383a
- Transformed into DH5α cells
- Plated BB-pRL1383a+J33207 on selective media with and without IPTG to verify if IPTG is necessary for blue/white screening in this strain
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
