Team:Hawaii/Notebook/2008-09-16

From 2008.igem.org

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Things we did today

Wetlab work

Plasmid prep

Grace
  • 5 ml plasmid prep of:
  • BB-pRL1383a
  • 3A: nir+rbs+slr1 #2, 3
  • 3A: nir+rbs+pilA #6
  • 3A: nir+rbs+slr1+GFPf #10
  • 3A: nir+rbs+pilA+GFPf12 # 2, 6
  • 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10

Gel Purification

Krystle
  • gel purified restriction digest products of:
    • nir
    • pilA
    • slr
    • pSB1A3
    • GFPf + tt #8
    • GFPf + tt #17
    • GFPf + tt #21

Gel Verify constructs

Gel used to verify the insertion of Promoter and RBS in front of aada(prl&bb).
Margaret
  • verified constructs: ap36, ab92, ab94, ab95, ab96, ab98
  • verified constructs will be gel purified and used in a ligation.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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