Alberta NINT/17 July 2008

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(New page: < Back to notebook < Previous entry lab work (SD): Since the double-plasmid cell cultures did grow on the LB...)
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             K102032+K102035 (LB+amp50+kan35)
             K102032+K102035 (LB+amp50+kan35)
             K102032+K102036 (LB+amp50+kan35)
             K102032+K102036 (LB+amp50+kan35)
 +
 +
lab work (JD):
 +
              Gel purification of pSB4A5/E+P, K102003/E+P, K102006/E+P
 +
              Quantitated the samples
 +
              Carried out ligations: K102003+pSB4A5=K102031, and K102006+pSB4A5=K102021
 +
              Transformed XL1-B Cells
 +
              Plated cells on LB+Amp plates
[[Alberta_NINT/18_July_2008 | Next entry >]]
[[Alberta_NINT/18_July_2008 | Next entry >]]

Revision as of 17:52, 21 July 2008

< Back to notebook

< Previous entry

lab work (SD): 

            Since the double-plasmid cell cultures did grow on the LB+kan35 plates we will try again to 
            grow them on LB+amp+kan35.  This time, 2.0ul of each of the plasmids is transformed into XL1-B 
            cells and plated in an attempt to increase transformation efficiency.
            K102032+K102011 (LB+amp50+kan35)
            K102032+K102034 (LB+amp50+kan35)
            K102032+K102035 (LB+amp50+kan35)
            K102032+K102036 (LB+amp50+kan35)

lab work (JD): 

             Gel purification of pSB4A5/E+P, K102003/E+P, K102006/E+P
             Quantitated the samples
             Carried out ligations: K102003+pSB4A5=K102031, and K102006+pSB4A5=K102021
             Transformed XL1-B Cells
             Plated cells on LB+Amp plates

Next entry >

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