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Alberta NINT/18 July 2008
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(New page: < Back to notebook < Previous entry lab work (SD): The double-plasmid cell cultures showed one-two colonies ...) |
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C 1.0 1.0 50 | C 1.0 1.0 50 | ||
D 2.0 2.0 50 | D 2.0 2.0 50 | ||
| + | |||
| + | lab work (JD): | ||
| + | Ligations seem to have worked: | ||
| + | K102031: 1 | ||
| + | K102021: 3 | ||
| + | Negative: 0 | ||
| + | Made O/Ns from these colonies | ||
| + | Replated cells left at the bottom of tubes from yesterday's transformation | ||
[[Alberta_NINT/21_July_2008 | Next entry >]] | [[Alberta_NINT/21_July_2008 | Next entry >]] | ||
Revision as of 17:56, 21 July 2008
lab work (SD):
The double-plasmid cell cultures showed one-two colonies on the LB+amp+kan35 plates.
To test transformation efficiency, we will plate 150ul of XL1-B with pSB4A5+pSB4K5 on LB+amp50,
LB+kan35, and LB+amp50+kan35 plates using different amounts of plasmid and cells.
pSB4A5 (ul) pSB4K5 (ul) XL1-B (ul)
A 1.0 1.0 100
B 4.0 4.0 100
C 1.0 1.0 50
D 2.0 2.0 50
lab work (JD):
Ligations seem to have worked:
K102031: 1
K102021: 3
Negative: 0
Made O/Ns from these colonies
Replated cells left at the bottom of tubes from yesterday's transformation
