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Alberta NINT/3 July 2008
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(New page: < Back to notebook < Previous entry lab work (SD): Sequenced pSB4A5 to verify. Made overnights f...) |
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Sequenced pSB4A5 to verify. | Sequenced pSB4A5 to verify. | ||
Made overnights from four colonies from each plate (K102022 and K102032) from 02/07/08. | Made overnights from four colonies from each plate (K102022 and K102032) from 02/07/08. | ||
| + | |||
| + | lab work (JD): | ||
| + | From sequencing K102006.4 becomes official K102006 | ||
| + | K102007.5 becomes official K102007 | ||
| + | Digested pSB4A5 with EcoR1 and Pst1 | ||
| + | Digested K102006 and K102007 with EcoR1 and Pst1 | ||
| + | Ran 2% agarose gel electrophoresis | ||
| + | Made glycerol stock of K102006 and K102007 | ||
[[Alberta_NINT/4_July_2008 | Next entry >]] | [[Alberta_NINT/4_July_2008 | Next entry >]] | ||
Revision as of 17:09, 9 July 2008
lab work (SD):
Sequenced pSB4A5 to verify.
Made overnights from four colonies from each plate (K102022 and K102032) from 02/07/08.
lab work (JD):
From sequencing K102006.4 becomes official K102006
K102007.5 becomes official K102007
Digested pSB4A5 with EcoR1 and Pst1
Digested K102006 and K102007 with EcoR1 and Pst1
Ran 2% agarose gel electrophoresis
Made glycerol stock of K102006 and K102007
