"
Edinburgh/18 September 2008
From 2008.igem.org
(Difference between revisions)
| Line 16: | Line 16: | ||
* M207~209 and P97 run on '''Gel 81'''. All failed: single and double digests yielded the same results for M207~209. PstI digest of P97 resulted in a smear. (AM) | * M207~209 and P97 run on '''Gel 81'''. All failed: single and double digests yielded the same results for M207~209. PstI digest of P97 resulted in a smear. (AM) | ||
* Transformation/plating of CHU1727 failed twice. (CKL) | * Transformation/plating of CHU1727 failed twice. (CKL) | ||
| + | |||
| + | === Follow-Up: Sequencing Results === | ||
| + | |||
| + | * M212 is pSB1A2-glgC triple mutant with desired prefix and suffix. | ||
| + | * M213 is NOT dxs+crtEBI. Both forward and reverse sequences indicate crtI. | ||
| + | * M218 is NOT dxs+crtEBI. Forward sequence is dxs; reverse sequence is gibberish. | ||
Revision as of 18:28, 6 October 2008
< Previous Entry | Notebook Index | Next Entry >
Week 14
Thursday 18 September ~ Friday 19 September 08
- Sent M213, M218 (dxs+crtEBI) for sequencing. (AM)
- Single (EcoRI) and double (EcoRI+PstI) digests of:
- M198 (SOB2+zm1)
- M201~203 (SOB2-rbs+zm1)
- M204~206 (SOB2+zm2)
- M207~209 (SOB2-rbs+zm2). (Yan)
- Single (PstI) digest of P97 (cex mut). (AM)
- M198~M206 run on Gel 80. All failed: single and double digests yielded the same results for all samples. (Yan)
- M207~209 and P97 run on Gel 81. All failed: single and double digests yielded the same results for M207~209. PstI digest of P97 resulted in a smear. (AM)
- Transformation/plating of CHU1727 failed twice. (CKL)
Follow-Up: Sequencing Results
- M212 is pSB1A2-glgC triple mutant with desired prefix and suffix.
- M213 is NOT dxs+crtEBI. Both forward and reverse sequences indicate crtI.
- M218 is NOT dxs+crtEBI. Forward sequence is dxs; reverse sequence is gibberish.
