"
Edinburgh/18 September 2008
From 2008.igem.org
(Difference between revisions)
(New page: <div id="header">{{Template:Team:Edinburgh/Templates/Header}}</div> '''< Previous Entry | Notebook Index | [[Edinburgh/16_Septe...) |
|||
| (2 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
<div id="header">{{Template:Team:Edinburgh/Templates/Header}}</div> | <div id="header">{{Template:Team:Edinburgh/Templates/Header}}</div> | ||
| - | '''[[Edinburgh/ | + | '''[[Edinburgh/17_September_2008|< Previous Entry]] | [[Team:Edinburgh/Notebook|Notebook Index]] | [[Edinburgh/Week_15|Next Entry >]]''' |
== Week 14 == | == Week 14 == | ||
=== Thursday 18 September ~ Friday 19 September 08 === | === Thursday 18 September ~ Friday 19 September 08 === | ||
| - | * Sent M213, M218 (dxs+crtEBI) for sequencing. (AM) | + | * Sent M213, M218 (''dxs+crtEBI'') for sequencing. (AM) |
* Single (EcoRI) and double (EcoRI+PstI) digests of: | * Single (EcoRI) and double (EcoRI+PstI) digests of: | ||
| - | ** M198 (SOB2+ | + | ** M198 (SOB2+''SU1'') |
| - | ** M201~203 (SOB2-rbs+ | + | ** M201~203 (SOB2-rbs+''SU1'') |
| - | ** M204~206 (SOB2+ | + | ** M204~206 (SOB2+''ISO2'') |
| - | ** M207~209 (SOB2-rbs+ | + | ** M207~209 (SOB2-rbs+''ISO2''). (Yan) |
| - | * Single (PstI) digest of P97 (cex mut). (AM) | + | * Single (PstI) digest of P97 (''cex'' mut). (AM) |
* M198~M206 run on '''Gel 80'''. All failed: single and double digests yielded the same results for all samples. (Yan) | * M198~M206 run on '''Gel 80'''. All failed: single and double digests yielded the same results for all samples. (Yan) | ||
* M207~209 and P97 run on '''Gel 81'''. All failed: single and double digests yielded the same results for M207~209. PstI digest of P97 resulted in a smear. (AM) | * M207~209 and P97 run on '''Gel 81'''. All failed: single and double digests yielded the same results for M207~209. PstI digest of P97 resulted in a smear. (AM) | ||
* Transformation/plating of CHU1727 failed twice. (CKL) | * Transformation/plating of CHU1727 failed twice. (CKL) | ||
| + | |||
| + | === Follow-Up: Sequencing Results === | ||
| + | |||
| + | * M212 is pSB1A2-''glgC16'' with desired prefix and suffix. | ||
| + | * M213 is NOT ''dxs+crtEBI''. Both forward and reverse sequences indicate ''crtI''. | ||
| + | * M218 is NOT ''dxs+crtEBI''. Forward sequence is dxs; reverse sequence is gibberish. | ||
Latest revision as of 22:29, 29 October 2008
< Previous Entry | Notebook Index | Next Entry >
Week 14
Thursday 18 September ~ Friday 19 September 08
- Sent M213, M218 (dxs+crtEBI) for sequencing. (AM)
- Single (EcoRI) and double (EcoRI+PstI) digests of:
- M198 (SOB2+SU1)
- M201~203 (SOB2-rbs+SU1)
- M204~206 (SOB2+ISO2)
- M207~209 (SOB2-rbs+ISO2). (Yan)
- Single (PstI) digest of P97 (cex mut). (AM)
- M198~M206 run on Gel 80. All failed: single and double digests yielded the same results for all samples. (Yan)
- M207~209 and P97 run on Gel 81. All failed: single and double digests yielded the same results for M207~209. PstI digest of P97 resulted in a smear. (AM)
- Transformation/plating of CHU1727 failed twice. (CKL)
Follow-Up: Sequencing Results
- M212 is pSB1A2-glgC16 with desired prefix and suffix.
- M213 is NOT dxs+crtEBI. Both forward and reverse sequences indicate crtI.
- M218 is NOT dxs+crtEBI. Forward sequence is dxs; reverse sequence is gibberish.
