User contributions
From 2008.igem.org
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
- 20:19, 29 October 2008 (diff | hist) N Utah State/28 October 2008 (New page: Cultures were inoculated in preparation for isolation of BioBricks tomorrow.) (top)
- 17:53, 24 October 2008 (diff | hist) Utah State/22 October 2008 (top)
- 17:52, 24 October 2008 (diff | hist) N Utah State/21 October 2008 (New page: Transformations were performed with yesterday's ligations.) (top)
- 17:51, 24 October 2008 (diff | hist) N Utah State/20 October 2008 (New page: Six ligations were performed, three with new T4 ligase and three with older T4 ligase: -35 Pro in pSB1A3, 5'F/SD R in pSB1A3, and phaB in pSB1A3. Cryostocks were made of 5'F/ATG R, 2x ...) (top)
- 17:48, 24 October 2008 (diff | hist) N Utah State/22 October 2008 (New page: PCR was performed for 5'F/SD R and -35F/ATG R and digested with E/S. This required a sequential digestion of Spe1 first then EcoR1 + additional 10X buffer O according to manufacturer's in...)
- 17:46, 24 October 2008 (diff | hist) Utah State/23 October 2008 (top)
- 17:45, 24 October 2008 (diff | hist) N Utah State/23 October 2008 (New page: Yesterday's PCR products were run out on a gel (5'F/SD R, -35F/ATG R), which showed that the PCR was unsuccessful. PCR was performed for two PHB promoter regions: 5'F/SD R and -35F/ATG...)
- 17:41, 24 October 2008 (diff | hist) N Utah State/24 October 2008 (New page: Took Joseph's PCR products from last night: 1. 5'F/SD R 2. -35F/ATG R 3. phaB F/R 4. phaC F/phaB R E/S digested #'s 1 and 2 E/P digested #'s 3 and 4 Ran out on a gel Cut out from ge...) (top)
- 01:31, 17 October 2008 (diff | hist) N Utah State/15 September 2008 (New page: Eight PCR reactions performed: phbCfor/rev, phbCfor/phbBrev, 5'CproFor/ATGrev, 5'CproFor/SDrev, 5'CproFor/TCrev, -35CproFor/ATGrev, -35phafor/TCrev, and a negative control. After electro...) (top)
- 23:50, 16 October 2008 (diff | hist) N Utah State/10 October 2008 (New page: Digested 9 samples of 4 biobricks E/P. Purified DNA of phbA in pSB3K3 and digested. Ran all samples on gel. Electrophoresis order in yellow book.) (top)
- 23:46, 16 October 2008 (diff | hist) N Utah State/11 October 2008 (New page: Restriction digest (E/P) was repeated on the 10 potential biobricks. They were reordered to put like biobricks next to each other: changed from: L,1,2,3,4,5,6,7,8,9,10,L to L,3,5,2,7,...) (top)
- 23:41, 16 October 2008 (diff | hist) Utah State/13 October 2008 (top)
- 23:41, 16 October 2008 (diff | hist) N Utah State/13 October 2008 (New page: 1. E/P digested Joseph's PCR product for phbB (#12 of his samples, lane 15 of his electrophoresis picture). 2. Make cultures of 10 possible biobrick samples to troubleshoot causes for m...)
- 23:39, 16 October 2008 (diff | hist) N Utah State/16 October 2008 (New page: Isolated 4 cultures of I13600, E/P digested (40 ul reactions), ran out on gel, cut our from gel (4 x pSB1A3). Performed PCR on 3 possible biobricks (#9, #4 and #7, #6 of 10 tubes of possi...) (top)
- 23:15, 8 October 2008 (diff | hist) N Utah State/8 October 2008 (New page: All liquid cultures from yesterday were turbid. 0.5 ml were saved for each possible biobrick for cryostocks. Plasmid DNA was isolated from the rest of each culture. Transformations were...) (top)
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)