User contributions
From 2008.igem.org
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- 20:19, 29 October 2008 (diff | hist) N Utah State/28 October 2008 (New page: Cultures were inoculated in preparation for isolation of BioBricks tomorrow.) (top)
- 17:53, 24 October 2008 (diff | hist) Utah State/22 October 2008 (top)
- 17:52, 24 October 2008 (diff | hist) N Utah State/21 October 2008 (New page: Transformations were performed with yesterday's ligations.) (top)
- 17:51, 24 October 2008 (diff | hist) N Utah State/20 October 2008 (New page: Six ligations were performed, three with new T4 ligase and three with older T4 ligase: -35 Pro in pSB1A3, 5'F/SD R in pSB1A3, and phaB in pSB1A3. Cryostocks were made of 5'F/ATG R, 2x ...) (top)
- 17:48, 24 October 2008 (diff | hist) N Utah State/22 October 2008 (New page: PCR was performed for 5'F/SD R and -35F/ATG R and digested with E/S. This required a sequential digestion of Spe1 first then EcoR1 + additional 10X buffer O according to manufacturer's in...)
- 17:46, 24 October 2008 (diff | hist) Utah State/23 October 2008 (top)
- 17:45, 24 October 2008 (diff | hist) N Utah State/23 October 2008 (New page: Yesterday's PCR products were run out on a gel (5'F/SD R, -35F/ATG R), which showed that the PCR was unsuccessful. PCR was performed for two PHB promoter regions: 5'F/SD R and -35F/ATG...)
- 17:41, 24 October 2008 (diff | hist) N Utah State/24 October 2008 (New page: Took Joseph's PCR products from last night: 1. 5'F/SD R 2. -35F/ATG R 3. phaB F/R 4. phaC F/phaB R E/S digested #'s 1 and 2 E/P digested #'s 3 and 4 Ran out on a gel Cut out from ge...) (top)
- 01:31, 17 October 2008 (diff | hist) N Utah State/15 September 2008 (New page: Eight PCR reactions performed: phbCfor/rev, phbCfor/phbBrev, 5'CproFor/ATGrev, 5'CproFor/SDrev, 5'CproFor/TCrev, -35CproFor/ATGrev, -35phafor/TCrev, and a negative control. After electro...) (top)
- 23:50, 16 October 2008 (diff | hist) N Utah State/10 October 2008 (New page: Digested 9 samples of 4 biobricks E/P. Purified DNA of phbA in pSB3K3 and digested. Ran all samples on gel. Electrophoresis order in yellow book.) (top)
- 23:46, 16 October 2008 (diff | hist) N Utah State/11 October 2008 (New page: Restriction digest (E/P) was repeated on the 10 potential biobricks. They were reordered to put like biobricks next to each other: changed from: L,1,2,3,4,5,6,7,8,9,10,L to L,3,5,2,7,...) (top)
- 23:41, 16 October 2008 (diff | hist) Utah State/13 October 2008 (top)
- 23:41, 16 October 2008 (diff | hist) N Utah State/13 October 2008 (New page: 1. E/P digested Joseph's PCR product for phbB (#12 of his samples, lane 15 of his electrophoresis picture). 2. Make cultures of 10 possible biobrick samples to troubleshoot causes for m...)
- 23:39, 16 October 2008 (diff | hist) N Utah State/16 October 2008 (New page: Isolated 4 cultures of I13600, E/P digested (40 ul reactions), ran out on gel, cut our from gel (4 x pSB1A3). Performed PCR on 3 possible biobricks (#9, #4 and #7, #6 of 10 tubes of possi...) (top)
- 23:15, 8 October 2008 (diff | hist) N Utah State/8 October 2008 (New page: All liquid cultures from yesterday were turbid. 0.5 ml were saved for each possible biobrick for cryostocks. Plasmid DNA was isolated from the rest of each culture. Transformations were...) (top)
- 21:06, 7 October 2008 (diff | hist) Utah State/6 October 2008 (top)
- 21:04, 7 October 2008 (diff | hist) N Utah State/7 October 2008 (New page: Liquid cultures were made from yesterday's colonies. Transformation will be done tomorrow. Trent) (top)
- 23:55, 6 October 2008 (diff | hist) N Utah State/3 October 2008 (New page: Ligation attempts were made for E0240 into pSB3K3.) (top)
- 23:54, 6 October 2008 (diff | hist) Utah State/6 October 2008
- 23:54, 6 October 2008 (diff | hist) N Utah State/6 October 2008 (New page: Ligations were attempted today for the phbA and phbB genes into the vectors pSB3K3 and pSB1A3. Transformations will be attempted Wednesday. Also, several ligations produced colonies from...)
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