User contributions
From 2008.igem.org
- 14:00, 19 August 2008 (diff | hist) Team:University of Ottawa/14 August 2008 (→Today in the Lab)
- 13:59, 19 August 2008 (diff | hist) N Team:University of Ottawa/14 August 2008 (New page: =='''Today in the Lab'''== '''Matt, Chris''' :<li> We performed a yeast transformation but made a mistake in the protocol so we are inoculating again tonight but this time only in Galacto...)
- 13:55, 19 August 2008 (diff | hist) N Team:University of Ottawa/13 August 2008 (New page: =='''Today in the Lab'''== '''Matt, Chris''' :<li> Performed PCR of PTP2 with SLN1 primers so that we have enough for yeast transformation. :<li> We inoculated both YPH500, YPH501 in gluc...)
- 14:17, 12 August 2008 (diff | hist) Team:University of Ottawa/11 August 2008
- 14:16, 12 August 2008 (diff | hist) Team:University of Ottawa/12 August 2008 (top)
- 14:16, 12 August 2008 (diff | hist) N Team:University of Ottawa/12 August 2008 (New page: {|######Navigational Bar####### |} <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=UTF-8" /> <title>Untitled Document</title>...)
- 14:14, 12 August 2008 (diff | hist) N Team:University of Ottawa/11 August 2008 (m)
- 14:11, 12 August 2008 (diff | hist) Team:University of Ottawa/10 August 2008 (→Today in the Lab) (top)
- 14:10, 12 August 2008 (diff | hist) N Team:University of Ottawa/10 August 2008 (New page: {|######Navigational Bar####### |} <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=UTF-8" /> <title>Untitled Document</title>...)
- 16:38, 8 August 2008 (diff | hist) Team:University of Ottawa/8 August 2008 (→Matt)
- 16:37, 8 August 2008 (diff | hist) Team:University of Ottawa/8 August 2008
- 16:35, 8 August 2008 (diff | hist) N Team:University of Ottawa/8 August 2008 (New page: {|######Navigational Bar####### |} <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=UTF-8" /> <title>Untitled Document</title>...)
- 16:34, 8 August 2008 (diff | hist) Team:University of Ottawa/7 August 2008 (→Matt)
- 16:34, 8 August 2008 (diff | hist) Team:University of Ottawa/7 August 2008 (→Chris)
- 19:10, 7 August 2008 (diff | hist) Team:University of Ottawa/31 July 2008 (→Matt)
- 19:01, 7 August 2008 (diff | hist) Team:University of Ottawa/31 July 2008 (→Chris)
- 18:54, 7 August 2008 (diff | hist) Team:University of Ottawa/30 July 2008 (→Matt)
- 18:53, 7 August 2008 (diff | hist) Team:University of Ottawa/30 July 2008 (→Chris)
- 18:52, 7 August 2008 (diff | hist) Team:University of Ottawa/29 July 2008 (→Matt) (top)
- 18:51, 7 August 2008 (diff | hist) Team:University of Ottawa/29 July 2008
- 18:47, 7 August 2008 (diff | hist) Team:University of Ottawa/28 July 2008 (top)
- 18:42, 7 August 2008 (diff | hist) Team:University of Ottawa/24 July 2008 (→Today in the lab)
- 18:41, 7 August 2008 (diff | hist) Team:University of Ottawa/24 July 2008 (→Today in the lab)
- 18:34, 7 August 2008 (diff | hist) Team:University of Ottawa/23 July 2008 (→Today in the lab) (top)
- 14:58, 25 July 2008 (diff | hist) N Team:University of Ottawa/22 July 2008 ([P)
- 15:05, 22 July 2008 (diff | hist) Team:University of Ottawa/21 July 2008 (→Today in the lab)
- 14:58, 22 July 2008 (diff | hist) Team:University of Ottawa/19 July 2008 (→Today in the lab)
- 18:41, 18 July 2008 (diff | hist) Team:University of Ottawa/18 July 2008 (→Today in the Lab)
- 18:40, 18 July 2008 (diff | hist) N Team:University of Ottawa/18 July 2008 (New page: __TOC__ ==Today in the Lab== '''Matt''' :'''PCR Amplification''' ::<li> A PCR Amplification was performed on 600/1 plasmids with integration primers so that yeast integration can be perfor...)
- 18:35, 18 July 2008 (diff | hist) Team:University of Ottawa/17 July 2008
- 18:28, 18 July 2008 (diff | hist) Team:University of Ottawa/16 July 2008
- 19:27, 16 July 2008 (diff | hist) Team:University of Ottawa/15 July 2008 (→Today on the Lab)
- 19:27, 16 July 2008 (diff | hist) Team:University of Ottawa/15 July 2008 (→Today on the Lab)
- 19:27, 16 July 2008 (diff | hist) Team:University of Ottawa/15 July 2008 (→Today on the Lab)
- 19:26, 16 July 2008 (diff | hist) Team:University of Ottawa/15 July 2008 (→Today on the Lab)
- 19:26, 16 July 2008 (diff | hist) Team:University of Ottawa/15 July 2008
- 19:18, 16 July 2008 (diff | hist) Team:University of Ottawa/15 July 2008
- 19:17, 16 July 2008 (diff | hist) Team:University of Ottawa/15 July 2008
- 19:14, 16 July 2008 (diff | hist) Team:University of Ottawa/14 July 2008 (→Today in the Lab)
- 19:13, 16 July 2008 (diff | hist) N Team:University of Ottawa/14 July 2008 (New page: __TOC__ ==Today in the Lab== '''Matt''' :'''PCR Amplification''' ::<li> Corey decided it would be best to start from the beginning and amplify the PTP2 out of Genomic DNA again. I redid th...)
- 19:02, 16 July 2008 (diff | hist) Team:University of Ottawa/11 July 2008
- 18:54, 16 July 2008 (diff | hist) N Team:University of Ottawa/11 July 2008 (New page: __TOC__ ==Today in the Lab== '''Matt''' :'''PTP2 Digestion''' ::<li> Ran two digestion's of the PTP2 in parallel with one another: AatII/NcoI, EcoRV/NcoI. Neither Gel gave proper band size...)
- 18:50, 16 July 2008 (diff | hist) Team:University of Ottawa/10 July 2008 (→Today in the lab)
- 18:39, 16 July 2008 (diff | hist) Team:University of Ottawa/9 July 2008
- 20:10, 8 July 2008 (diff | hist) Team:University of Ottawa/8 July 2008 (→Today in the Lab=)
- 20:09, 8 July 2008 (diff | hist) N Team:University of Ottawa/8 July 2008 (New page: __TOC__ ==Today in the Lab=== '''Matt''' :'''Digestion''' ::<li> Digestion was performed again with PstI for PTP2 ligation product.\, this time longer digestion period with more enzyme. ::...)
- 20:56, 7 July 2008 (diff | hist) Team:University of Ottawa/7 July 2008 (→Today in the lab)
- 17:44, 7 July 2008 (diff | hist) N Team:University of Ottawa/4 July 2008 (New page: __TOC__ ==Today in the Lab== :'''Matt''' :'''Miniprep''' ::<li> Inoculation turned out great from the night before. ::<li> A miniprep was performed on all the plasmids from the inoculation...)
- 17:32, 3 July 2008 (diff | hist) Team:University of Ottawa/3 July 2008 (→Today in the Lab)
- 17:31, 3 July 2008 (diff | hist) Team:University of Ottawa/3 July 2008 (→Today in the Lab)
- 16:20, 3 July 2008 (diff | hist) Team:University of Ottawa/3 July 2008 (→Today in the Lab)
- 16:20, 3 July 2008 (diff | hist) N Team:University of Ottawa/3 July 2008 (New page: __TOC__ ==Today in the Lab== '''Matt''' :'''Competent Cells''' ::<li> Ecoli integrated with ligation products were successful in growing overnight however Atcre did not show any signs of g...)
- 15:11, 3 July 2008 (diff | hist) Team:University of Ottawa/2 July 2008 (→Today in the Lab)
- 15:10, 3 July 2008 (diff | hist) Team:University of Ottawa/2 July 2008 (→Today in the Lab)
- 15:05, 3 July 2008 (diff | hist) N Team:University of Ottawa/2 July 2008 (New page: __TOC__ ==Today in the Lab== '''Matt''' :'''Ligation''' ::<li> I am using 2:1, 3:1, 4:1 molar ratio of insert to vector being used for ligation of PTP2 to pSSA42. ::<li> A gel confirmation...)
- 13:50, 26 June 2008 (diff | hist) N Team:University of Ottawa/25 June 2008 (New page: __TOC__ ==Today in the Lab== '''Matt''' :'''PCR Amplification''' ::<li> F60, F61 primers used for PTP2 cassette. I also labelled all the primers we received that we will be using for the n...)
- 13:42, 26 June 2008 (diff | hist) Team:University of Ottawa/24 June 2008 (→Today in the Lab)
- 13:41, 26 June 2008 (diff | hist) N Team:University of Ottawa/24 June 2008 (New page: __TOC__ ==Today in the Lab== '''Matt''' :'''Genomic DNA extraction''' ::<li> Mila showed me how to do a genomic DNA extraction of the four YPH500 colonies. ::<li> Absorbance of colony A wh...)
- 13:35, 26 June 2008 (diff | hist) N Team:University of Ottawa/23 June 2008 (New page: __TOC__ ==Today in the Lab== '''Matt''' :'''Inoculation''' :<li>Inoculation was performed using both a streaked plate and liquid media using the YPH500 strain of yeast. :<li>Four colonies ...)
- 15:52, 23 June 2008 (diff | hist) Team:University of Ottawa/20 June 2008 (→Today in the Lab)
- 15:51, 23 June 2008 (diff | hist) Team:University of Ottawa/20 June 2008 (→Today in the Lab)
- 15:14, 23 June 2008 (diff | hist) N Team:University of Ottawa/20 June 2008 (New page: __TOC__ ==Today in the Lab== '''Glycerol Stock and Transformation''' '''Dan, Matt''' :<li>A glycerol stock of YPH500 using 0.5 mL cells and 0.5 mL YPD was made. :<li>Transformation's of th...)
- 15:07, 23 June 2008 (diff | hist) Team:University of Ottawa/17 June 2008 (→Today in the Lab)
- 19:24, 18 June 2008 (diff | hist) Team:University of Ottawa/17 June 2008 (→Today in the Lab)
- 19:23, 18 June 2008 (diff | hist) N Team:University of Ottawa/17 June 2008 (New page: __TOC__ =='''Today in the Lab'''== ''')
- 19:21, 18 June 2008 (diff | hist) Team:University of Ottawa/16 June 2008 (→Today in the Lab)
- 18:34, 18 June 2008 (diff | hist) Team:University of Ottawa/16 June 2008 (→Today in the Lab)
- 18:33, 18 June 2008 (diff | hist) Team:University of Ottawa/16 June 2008
- 17:02, 18 June 2008 (diff | hist) Team:University of Ottawa/16 June 2008 (→Today in the Lab)
- 17:01, 18 June 2008 (diff | hist) Team:University of Ottawa/16 June 2008
- 16:54, 18 June 2008 (diff | hist) Team:University of Ottawa/13 June 2008 (→General project developments)
- 16:54, 18 June 2008 (diff | hist) Team:University of Ottawa/13 June 2008 (→General project developments)
- 16:54, 18 June 2008 (diff | hist) Team:University of Ottawa/13 June 2008
- 16:50, 18 June 2008 (diff | hist) Team:University of Ottawa/13 June 2008
- 16:46, 18 June 2008 (diff | hist) Team:University of Ottawa/14 June 2008 (→Today in the lab)
- 16:45, 18 June 2008 (diff | hist) Team:University of Ottawa/16 June 2008
- 16:45, 18 June 2008 (diff | hist) N Team:University of Ottawa/16 June 2008 (New page: __TOC__ 'Today in the Lab' ::Dan, Matt, Corey)
- 19:50, 11 June 2008 (diff | hist) Team:University of Ottawa/26 May 2008 (→Today)
- 19:44, 11 June 2008 (diff | hist) N Team:University of Ottawa/11 June 2008 (New page: __TOC__ ==Today in the Lab== '''Dan, Matt''' :'''Gel Electrophoresis + PCR''' ::<li> Plasmids 7 + 8 were unsuccessful upon verifying with the Gel. The bands did not appear in the correct l...)
- 19:32, 11 June 2008 (diff | hist) Team:University of Ottawa/10 June 2008 (→Today in the Lab)
- 19:31, 11 June 2008 (diff | hist) Team:University of Ottawa/10 June 2008 (→Today in the Lab)
- 19:29, 11 June 2008 (diff | hist) Team:University of Ottawa/10 June 2008 (→Today in the Lab)
- 19:29, 11 June 2008 (diff | hist) Team:University of Ottawa/10 June 2008 (→Today in the Lab)
- 19:27, 11 June 2008 (diff | hist) Team:University of Ottawa/10 June 2008
- 19:26, 11 June 2008 (diff | hist) Team:University of Ottawa/10 June 2008
- 19:26, 11 June 2008 (diff | hist) Team:University of Ottawa/10 June 2008
- 19:24, 11 June 2008 (diff | hist) N Team:University of Ottawa/10 June 2008 (New page: __TOC__ '''Today in the Lab''' :'''Primers''' ::<li>)
- 19:21, 11 June 2008 (diff | hist) Team:University of Ottawa/6 June 2008 (→Today in the lab)
- 19:20, 11 June 2008 (diff | hist) N Team:University of Ottawa/9 June 2008 (New page: __TOC__)
- 18:33, 6 June 2008 (diff | hist) Team:University of Ottawa/6 June 2008 (→Today in the lab)
- 18:31, 6 June 2008 (diff | hist) Team:University of Ottawa/4 June 2008 (→Today in the lab)
- 18:28, 6 June 2008 (diff | hist) Team:University of Ottawa/6 June 2008 (→Today in the lab)
- 18:26, 6 June 2008 (diff | hist) Team:University of Ottawa/5 June 2008 (→Today in the Lab)
- 18:26, 6 June 2008 (diff | hist) Team:University of Ottawa/5 June 2008 (→Today in the Lab)
- 18:25, 6 June 2008 (diff | hist) Team:University of Ottawa/5 June 2008 (→Today in the Lab)
- 18:22, 6 June 2008 (diff | hist) Team:University of Ottawa/5 June 2008 (→Today in the Lab)
- 18:20, 6 June 2008 (diff | hist) Team:University of Ottawa/6 June 2008 (→Today in the lab)
- 18:20, 6 June 2008 (diff | hist) N Team:University of Ottawa/6 June 2008 (New page: ==Today in the lab== '''Dan, Matt''' ::<li> Miniprep continued form yesterday with the 6 colonies chosen. ::<li> Upon determining concentrations of DNA, we had a few colonies with a very l...)
- 18:16, 6 June 2008 (diff | hist) N Team:University of Ottawa/5 June 2008 (New page: ==Today in the Lab== '''Dan, Matt''' ::<li>We decided to use different enzymes - Bam HI + EcoRI for 8/7 and xhoI for 0/1 ::<li>Gel was unsuccessful for 1A ::<li>We decided to choose 6 new ...)
- 18:05, 6 June 2008 (diff | hist) Team:University of Ottawa/4 June 2008 (→Today in the lab)
- 18:04, 6 June 2008 (diff | hist) N Team:University of Ottawa/4 June 2008 (New page: ==Today in the lab== '''Dan, Matt''' ::<li>Miniprep was performed for all four plasmids and concentrations of DNA were calculated.)
- 19:24, 27 May 2008 (diff | hist) Team:University of Ottawa/27 May 2008 (→Today in the Lab)
- 19:13, 27 May 2008 (diff | hist) Team:University of Ottawa/27 May 2008 (→Today in the Lab)
- 19:11, 27 May 2008 (diff | hist) Team:University of Ottawa/27 May 2008 (→Today in the Lab)
- 19:10, 27 May 2008 (diff | hist) Team:University of Ottawa/27 May 2008
- 19:01, 27 May 2008 (diff | hist) N Team:University of Ottawa/27 May 2008 (New page: __TOC__ ==Today in the Lab== =Tripel Digestion completed for the first plasmid=)