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Team:BCCS-Bristol/Notebook
From 2008.igem.org
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***but unfortunenately, no chemotaxis was observed | ***but unfortunenately, no chemotaxis was observed | ||
| + | * [[Team:BCCS-Bristol/Notebook_Progress# 7th August- 13th August|'''7th August- 13th August''']] | ||
| + | ** BioBrick Transformation | ||
| + | ***The self-made electro competent ''E. coli'' DH5α cells are working | ||
| + | ***A longer incubation time of the punched paper disc in water seems to resolve more BioBrick DNA | ||
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Revision as of 18:00, 18 August 2008
  
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Lab Notebook
This section holds our team lab notebook which can be accessed by date. Click on any of the red dates to see what we got up to.
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Protocols
Preparation of electro competent cells
Transformation with heat shock
Transformation using electroporation
Progress Reports
- 30th July- 6th August
- BioBrick Transformation
- Heat shock transformations with BioBrick DNA resulted in zero, one or two colonies per attempt
- All colonies have been verified to possess the plasmids
- Electroporation is much more successful
- Agar plug assay
- E. coli MG1655 moved apparently some beads!!!
- but unfortunenately, no chemotaxis was observed
- BioBrick Transformation
- 7th August- 13th August
- BioBrick Transformation
- The self-made electro competent E. coli DH5α cells are working
- A longer incubation time of the punched paper disc in water seems to resolve more BioBrick DNA
- BioBrick Transformation
