Team:BCCS-Bristol/Notebook

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Revision as of 11:49, 19 August 2008

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This section holds our team lab notebook which can be accessed by date. Click on any of the red dates to see what we got up to.

Since August, we started to make a weekly summaries of our lab work progress. You find them on our Progress_Page.

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 * [[Team:BCCS-Bristol/Protocols-Transformation_with_heat_shock|'''Transformation with heat shock''']]
 * [[Team:BCCS-Bristol/Protocols-Transformation_using_electroporation|'''Transformation using electroporation''']]
-===Progress Reports===
-* [[Team:BCCS-Bristol/Notebook_Progress# 30th July- 6th August|'''30th July- 6th August''']]
-** BioBrick Transformation
-*** Heat shock transformations with BioBrick DNA resulted in zero, one or two colonies per attempt
-*** All colonies have been verified to possess the plasmids
-*** Electroporation is much more successful
-** Agar plug assay
-***''E. coli'' MG1655 moved apparently some beads!!!
-***but unfortunenately, no chemotaxis was observed
-* [[Team:BCCS-Bristol/Notebook_Progress# 7th August- 13th August|'''7th August- 13th August''']]
-** BioBrick Transformation
-***The self-made electro competent ''E. coli'' DH5α cells are working
-***A longer incubation time of the punched paper disc in water seems to resolve more BioBrick DNA
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