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Team:BCCS-Bristol/Notebook
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Since August, we started to make a weekly summaries of our lab work progress. You find them on our [[Team:BCCS-Bristol/Notebook-Progress|Progress Page]]. | Since August, we started to make a weekly summaries of our lab work progress. You find them on our [[Team:BCCS-Bristol/Notebook-Progress|Progress Page]]. | ||
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===Protocols=== | ===Protocols=== | ||
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* [[Team:BCCS-Bristol/Protocols-Testing_the_activity_of_biobrick_T9002|'''Testing the activity of biobrick T9002''']] | * [[Team:BCCS-Bristol/Protocols-Testing_the_activity_of_biobrick_T9002|'''Testing the activity of biobrick T9002''']] | ||
<br> | <br> | ||
| + | ===Progress Reports=== | ||
| + | |||
| + | * [[Team:BCCS-Bristol/Notebook-Progress#1st_July_-_8th July|'''1st July - 8th July ''']] | ||
| + | ** Swimming agar assay | ||
| + | |||
| + | * [[Team:BCCS-Bristol/Notebook-Progress#9th_July_-_15th July|'''9th July - 15th July ''']] | ||
| + | |||
| + | * [[Team:BCCS-Bristol/Notebook-Progress#16th_July_-_22nd July|'''16th July - 22nd July ''']] | ||
| + | |||
| + | * [[Team:BCCS-Bristol/Notebook-Progress#23rd_July_-_29th July|'''23rd July - 29th July ''']] | ||
| + | |||
| + | * [[Team:BCCS-Bristol/Notebook-Progress#30th_July_-_6th August|'''31st July - 6th August''']] | ||
| + | ** BioBrick Transformation | ||
| + | *** Heat shock transformations with BioBrick DNA resulted in zero, one or two colonies per attempt | ||
| + | *** All colonies have been verified to possess the plasmids | ||
| + | *** Electroporation is much more successful | ||
| + | ** Agar plug assay | ||
| + | ***''E. coli'' MG1655 moved apparently some beads!!! | ||
| + | ***but unfortunenately, no chemotaxis was observed | ||
| + | |||
| + | * [[Team:BCCS-Bristol/Notebook-Progress#7th_August_-_13th August|'''7th August - 13th August''']] | ||
| + | ** BioBrick Transformation | ||
| + | ***The self-made electro competent ''E. coli'' DH5α cells are working | ||
| + | ***A longer incubation time of the punched paper disc in water seems to resolve more BioBrick DNA | ||
| + | |||
| + | * [[Team:BCCS-Bristol/Notebook-Progress#14th_August_-_20th August|'''14th August - 20th August''']] | ||
| + | ** Agar plug assay | ||
| + | *** Repulsion achieved to some degree in the agar-plug assay. | ||
| + | ** BioBrick transformation | ||
| + | *** DH5α transformed with pRZ1(pRS415 containing cpxR responsive promotor - sent to us by T.Silhavy, Princeton) | ||
| + | |||
| + | * [[Team:BCCS-Bristol/Notebook-Progress#2nd_September_-_9th September|'''2nd September - 9th September''']] | ||
| + | ** BioBrick transformation | ||
| + | |||
| + | * [[Team:BCCS-Bristol/Notebook-Progress#10th_September_-_16th September|'''10th September - 16th September''']] | ||
| + | |||
| + | |||
| + | |||
</div> | </div> | ||
Revision as of 15:34, 23 September 2008
  
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Lab Notebook
This section holds our team lab notebook which can be accessed by date. Click on any of the red dates to see what we got up to.
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Since August, we started to make a weekly summaries of our lab work progress. You find them on our Progress Page.
Protocols
- Agarose Gel Electrophoresis
- Colony PCR
- DMSO stocks
- Preparation of electro competent cells
- Media
- Resuspending Primers
- Transformation with heat shock
- Transformation using electroporation
- Testing the activity of biobrick T9002
Progress Reports
- 1st July - 8th July
- Swimming agar assay
- 31st July - 6th August
- BioBrick Transformation
- Heat shock transformations with BioBrick DNA resulted in zero, one or two colonies per attempt
- All colonies have been verified to possess the plasmids
- Electroporation is much more successful
- Agar plug assay
- E. coli MG1655 moved apparently some beads!!!
- but unfortunenately, no chemotaxis was observed
- BioBrick Transformation
- 7th August - 13th August
- BioBrick Transformation
- The self-made electro competent E. coli DH5α cells are working
- A longer incubation time of the punched paper disc in water seems to resolve more BioBrick DNA
- BioBrick Transformation
- 14th August - 20th August
- Agar plug assay
- Repulsion achieved to some degree in the agar-plug assay.
- BioBrick transformation
- DH5α transformed with pRZ1(pRS415 containing cpxR responsive promotor - sent to us by T.Silhavy, Princeton)
- Agar plug assay
- 2nd September - 9th September
- BioBrick transformation
