Team:BCCS-Bristol/Notebook

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Lab Notebook

This section holds our team lab notebook which can be accessed by date. Click on any of the red dates to see what we got up to.

July
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31
August
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
September
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
October
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31

Protocols

Agarose Gel Electrophoresis

Colony PCR

DMSO stocks

Preparation of electro competent cells

Media

Resuspending Primers

Transformation with heat shock

Transformation using electroporation

Progress Reports

  • 30th July- 6th August
    • BioBrick Transformation
      • Heat shock transformations with BioBrick DNA resulted in zero, one or two colonies per attempt
      • All colonies have been verified to possess the plasmids
      • Electroporation is much more successful
    • Agar plug assay
      • E. coli MG1655 moved apparently some beads!!!
      • but unfortunenately, no chemotaxis was observed



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