Team:BCCS-Bristol/Notebook
From 2008.igem.org
Lab Notebook
This section holds our team lab notebook which can be accessed by date. Click on any of the red dates to see what we got up to.
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Since August, we started to make a weekly summaries of our lab work progress. You find them on our Progress_Page.
Protocols
- Agarose Gel Electrophoresis
- Colony PCR
- DMSO stocks
- Preparation of electro competent cells
- Media
- Resuspending Primers
- Transformation with heat shock
- Transformation using electroporation
Progress Reports
- 30th July- 6th August
- BioBrick Transformation
- Heat shock transformations with BioBrick DNA resulted in zero, one or two colonies per attempt
- All colonies have been verified to possess the plasmids
- Electroporation is much more successful
- Agar plug assay
- E. coli MG1655 moved apparently some beads!!!
- but unfortunenately, no chemotaxis was observed
- BioBrick Transformation
- 7th August- 13th August
- BioBrick Transformation
- The self-made electro competent E. coli DH5α cells are working
- A longer incubation time of the punched paper disc in water seems to resolve more BioBrick DNA
- BioBrick Transformation