Team:BCCS-Bristol/Notebook
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Lab Notebook
This section holds our team lab notebook which can be accessed by date. Click on any of the red dates to see what we got up to.
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Since August, we started to make a weekly summaries of our lab work progress. You find them on our Progress Page.
Protocols
- Agarose Gel Electrophoresis
- Colony PCR
- DMSO stocks
- Preparation of electro competent cells
- Media
- Resuspending Primers
- Transformation with heat shock
- Transformation using electroporation
- Testing the activity of biobrick T9002
Progress Reports
- 1st July - 8th July
- Swimming agar assay
- 31st July - 6th August
- BioBrick Transformation
- Heat shock transformations with BioBrick DNA resulted in zero, one or two colonies per attempt
- All colonies have been verified to possess the plasmids
- Electroporation is much more successful
- Agar plug assay
- E. coli MG1655 moved apparently some beads!!!
- but unfortunenately, no chemotaxis was observed
- BioBrick Transformation
- 7th August - 13th August
- BioBrick Transformation
- The self-made electro competent E. coli DH5α cells are working
- A longer incubation time of the punched paper disc in water seems to resolve more BioBrick DNA
- BioBrick Transformation
- 14th August - 20th August
- Agar plug assay
- Repulsion achieved to some degree in the agar-plug assay.
- BioBrick transformation
- DH5α transformed with pRZ1(pRS415 containing cpxR responsive promotor - sent to us by T.Silhavy, Princeton)
- Agar plug assay
- 2nd September - 9th September
- BioBrick transformation