Team:Bologna/Parts
From 2008.igem.org
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| [http://partsregistry.org/Part:BBa_K079024 K079024] | | [http://partsregistry.org/Part:BBa_K079024 K079024] | ||
- | | LacI repressor under the control of the J23118 | + | | LacI repressor under the control of the J23118 costitutive promoter and Lac 1 operator |
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | | [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2 (HC)] | ||
| [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079024 1243] | | [https://2008.igem.org/Team:Bologna/Parts/GelBBa_K079024 1243] |
Revision as of 17:53, 29 October 2008
HOME | PROJECT | TEAM | SOFTWARE | MODELING | WET LAB | LAB-BOOK | SUBMITTED PARTS | BIOSAFETY AND PROTOCOLS |
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Composites
The table below shows all the composites that were obtained and use to build up our final device. For each part, it ispossible to find:
-the link to the Registry in the second column ;
-part components, in the third column ;
-in the fourth column the plasmid the part has been cloned in;
-in the fifth the insert length after double digestion with Xba1/Pst1 enzymes linked to the electrophoresis gel run;
-in the sixth column cell vitality after transformation;
-in the seventh column fluo data for each part.
N° | Igem Code | Fragment | Plasmid | Length (bp) | Vitality | Fluo |
| K079015 | LacY transporter protein from E. coli (for the improvement of the registry) | pSB1AK3 (HC) | 1254 | | |
| K079017 | Lac symmetric - operator library member | GENEART pGA18 | 20 | | |
| K079018 | Lac 1 - operator library member | GENEART pGA18 | 21 | | |
| K079019 | Lac 2 - operator library member | GENEART pGA18 | 21 | | |
| K079036 | Tet O operator library member | GENEART pMA | 15 | | |
| K079037 | TetO-4C - operator library member | GENEART pMA | 15 | | |
| K079038 | TetO-wt/4C5G - operator library member | GENEART pMA | 15 | | |
| K079039 | LexA 1 - operator library member | GENEART pMA | 16 | | |
| K079040 | LexA 2 - opeartor library member | GENEART pMA | 16 | | |
| K079041 | Lambda OR1 - operator library member | GENEART pMA | 17 | | |
| K079042 | Lambda OR2 - operator library member | GENEART pMA | 17 | | |
| K079043 | Lambda OR3 - operator library member | GENEART pMA | 17 | | |
| K079045 | Lac operator library | GENEART pGA18 | 78 | | |
| K079046 | Tet operator library | GENEART pMA | 61 | | |
| K079047 | Lambda operator library | GENEART pMA | 67 | | |
| K079048 | LexA operator library | GENEART pMA | 40 | | |
| K079020 | LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 2 operator | pSB1A2 (HC) | 2165 | | |
| K079026 | GFP reporter protein and LacI gene sequence controlled by J23118 constitutive promoter | pSB1A2 (HC) | 2116 | | |
| K079029 | GFP expression controlled by LacI repressor under the BBa_J23114 promoter | pSB1A2 (HC) | 2501 | | |
| K079049 | GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator | pSB1A2 (HC) | 981 | | |
| K079050 | GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator | pSB1A2 (HC) | 981 | | |
| K079023 | LacI repressor under the control of the J23118 constitutive promoter and Lac 2 operator | pSB1A2 (HC) | 1243 | | |
| K079024 | LacI repressor under the control of the J23118 costitutive promoter and Lac 1 operator | pSB1A2 (HC) | 1243 | | |
| K079030 | GFP reporter protein controlled by the BBa_23105 constitutive promoter | pSB1A2 (HC) | 957 | | |
| K079031 | GFP reporter protein under the control of the BBa_J23118 promoter | pSB1A2 (HC) | 957 | | |
| K079032 | GFP reporter protein under the control of the BBa_J23100 constitutive promoter | pSB1A2 (HC) | 957 | | |