Team:Chiba/Calendar-Home/12 September 2008

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(Team:Input)
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*Pbad/araC:L-Arabinose induction test
*Pbad/araC:L-Arabinose induction test
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*BBa_I1304(Ptet-RBS-GFP,XL10G)×1()(ネガティブコントロール用)
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*[http://partsregistry.org/Part:BBa_I1304 BBa_I1304]×1(1)(negative control)
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*BBa_R0051(PcI)×4(②~⑤)のコロニーをつついて2ml培養。
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*[http://partsregistry.org/Part:BBa_R0051 BBa_R0051]×4(1)~(5)Select single colony from Agar Plate and introduce to 2mlLB each other.
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#incubated cultures at 37 degrees for 12hours.
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#incubated cultures at 37 &degC; for 12hours.
#Measured OD<sub>600</sub>.
#Measured OD<sub>600</sub>.
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Reaction mixtures were mixed in 48 well plate(deep well).
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Reaction mixtures were mixed in 48-well plate(deep well).
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混ぜ表
 
<table width="257" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="257" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
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<td></td><td></td><td> </td><td> </td><td> </td>
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<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td>
<tr>
<tr>
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<td>Cultures(μL)</td>
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<td>Cultures(&mu;L)</td>
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<td>0μl</td><td>1.9μl</td><td>19μl</td><td>190μl</td><td>-</td>
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<td>0&mu;l</td><td>1.9&mu;l</td><td>19&mu;l</td><td>190&mu;l</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
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<td> 20%L-Arabinose(μL)</td>
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<td> 20%L-Arabinose(&mu;L)</td>
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<td>0μl</td><td>1.9μl</td><td>19μl</td><td>190μl</td><td>-</td>
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<td>0&mu;l</td><td>1.9&mu;l</td><td>19&mu;l</td><td>190&mu;l</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
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</table>
</table>
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L-アラビノースを入れてから15min,1h,2h,6h,12h,24h後に蛍光強度を計測した。
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Measured the Green Fluorescence intensity by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6).
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Add L-arabinose and 30min,1h,2h,6h,12h,24h later, we measured intensicty of fluorescence.
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Result:
Result:
<table width="400" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="400" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<td width="2300">Time after arabinose induction  / Sample No.</td>
<td width="2300">Time after arabinose induction  / Sample No.</td>
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<td></td><td></td><td></td><td></td><td></td>
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<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td>
<tr>
<tr>
<td>30min</td>
<td>30min</td>

Latest revision as of 21:26, 29 October 2008

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11 September 2008 <|> 13 September 2008

Contents

Laboratory work

Team:Input

  • Pbad/araC:L-Arabinose induction test
  • [http://partsregistry.org/Part:BBa_I1304 BBa_I1304]×1(1)(negative control)
  • [http://partsregistry.org/Part:BBa_R0051 BBa_R0051]×4(1)~(5)Select single colony from Agar Plate and introduce to 2mlLB each other.


  1. incubated cultures at 37 &degC; for 12hours.
  2. Measured OD600.


Reaction mixtures were mixed in 48-well plate(deep well).


Sample No. 12345
Cultures(μL) 0μl1.9μl19μl190μl-
20%L-Arabinose(μL) 0μl1.9μl19μl190μl-
L-Arabinase conc. 0%(N.C)0.2%2%20%P.C

Measured the Green Fluorescence intensity by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6). Add L-arabinose and 30min,1h,2h,6h,12h,24h later, we measured intensicty of fluorescence. Result:

Time after arabinose induction / Sample No. 12345
30min 8.8909.75210.109.4139.701
1h 9.16113.9317.3714.9710.60
2h 9.41727.9640.2529.8710.72
6h 9.93374.99150.9125.010.04
12h 10.6499.39202.8872.0811.76
24h 14.66105.6447.333.3117.11

Team:Communication

no work

Team:Output

no work