Team:Chiba/Calendar-Home/13 September 2008

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12 September 2008 <|> 14 September 2008

Contents

Laboratory work

Team:Input

Time Delay Test

receiver

  1. [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908)


sender

  1. Plac-RhlI (JW1908)-Amp
  2. Plac-LasI (JW1908)-Amp
  3. Ptet-LuxI (JW1908)-Amp


48-well plate(Deep Well)

1:2ml 2:2ml,AHL2:2ml3:2ml4:2ml-
- -----
1:1ml,2:1ml,IPTG:2μl 1:100μl,2:1ml,IPTG:1.1μl110μl,21ml,IPTG:101μl1:1μl,21ml,IPTG:1.001μl1:1ml,2:100μl,IPTG:1.1μl1:1ml,2:10μl,IPTG:1.01μl
- -----
1:1ml,3:1ml,IPTG:2μl 1:100μl,3:1ml,IPTG:1.1μl1:10μl,3:1ml,IPTG:1.01μl1:1μl,3:1ml,IPTG1:1ml,3:100μl,IPTG:1.1μl1:1ml,3:10μl,IPTG:1.01μl
- -----
1:1ml,4:1ml,IPTG:2μl 1:100μl,4:1ml,IPTG:1.1μl1:10μl,4:1ml,IPTG:1.0μl1:1μl,4:1ml,IPTG:1.001μl1:1ml,4:100μl,IPTG:1.0μl1:1ml,4:10μl,IPTG1.01μl
- -----




Team:Communication

Gel Check
  1. [http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
  2. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
  3. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
Chiba-0913.JPG
Sample No. 1~3
Sample DNA(μL) 1
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
From left;
  1. [http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
  3. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)


-->Digestion

  1. [http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
  3. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)


Sample No. 1234
Sample DNA(μL) 5511
EcoRI(μL) 0.5o.5--
SpeI(μL) 0.50.5--
Buffer 1(μL) 11--
Buffer 3(μL) --1-
BSA(μL) 11--
dH2O(μL) 227.5-
TOTAL(μL) 1010101


--->(9/14)[[1]]

Team:Output

no work