From 2008.igem.org
(Difference between revisions)
|
|
| Line 58: |
Line 58: |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <td>EcoRⅠ</td><td>0.05</td><td>0.05</td> | + | <td>EcoRI</td><td>0.05</td><td>0.05</td> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| - | <td>PstⅠ</td><td>-</td><td>0.05</td> | + | <td>PstI</td><td>-</td><td>0.05</td> |
| | </tr> | | </tr> |
| | <tr> | | <tr> |
| Line 131: |
Line 131: |
| | | | |
| | :--->(5/9)'''Liquid Culture''' | | :--->(5/9)'''Liquid Culture''' |
| - |
| |
| - |
| |
| | | | |
| | ===Team:Output=== | | ===Team:Output=== |
Latest revision as of 21:04, 29 October 2008
>Home | Notebook
3 September 2008 <|> 5 September 2008
Laboratory work
Team:Input
no work
Team:Communication
- --->Gel Check
|
| Sample DNA |
1 |
| Loading Dye |
1 |
| dH2O |
4 |
| TOTAL |
6μL |
- From left;
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007) -> OK
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007) -> OK
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006) -> OK
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006) -> OK
|
|
- From left;
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0261](2007) -> OK
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0261](2007) -> OK
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0261](2006) -> OK
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0261](2006) -> OK
-
|
- --->Digestion test
| Sample |
Single Digesiton | Double Digestion |
| Sample DNA | 1 | 2 |
| EcoRI | 0.05 | 0.05 |
| PstI | - | 0.05 |
| Buffer 3 |
1 | 1 |
| BSA |
1 | 1 |
| dH2O |
6.95 | 5.9 |
| TOTAL |
10μL | 10μL |
- --->Gel Check
| Sample DNA |
10 |
| Loading Dye |
2 |
| TOTAL |
12μL |
|
- From left;
- Single Digestion
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007)
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007)
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006)
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006)
- [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2007)
- [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2007)
- [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2006)
- [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2006)
- ladder
- Double digestion
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007)
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007)
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006)
- [http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006)
- [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2007)
- [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2007)
- [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2006)
- [http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2006)
|
- Transformation
- Competent cells : JW1908
- Transformed the following and grew on new ampicillin plates.
- [http://partsregistry.org/Part:BBa_K084009 BBa_K084009](R-3,4,6,7)
- [http://partsregistry.org/Part:BBa_K084010 BBa_K084010](C-1,2,6,7)
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
- --->(5/9)Liquid Culture
Team:Output
Transformation
- [http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x3
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003] x3
"
