Team:Chiba/Calendar-Home/5 September 2008

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[[Team:Chiba/protocol/gelcheck|Gel Check]]
[[Team:Chiba/protocol/gelcheck|Gel Check]]

Revision as of 19:12, 29 October 2008

>Home | Notebook

4 September 2008 <|> 6 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(4/9)--->Liquid Culture
Cultured the following cells (2mL LB-Amp, at 37°C)
from transformed plates:
  • [http://partsregistry.org/Part:BBa_K084009 BBa_K084009](Plac+RBS+RhlI+LVA, Competent Cells : JW1908)(r1, r3~r7)
  • [http://partsregistry.org/Part:BBa_K084010 BBa_K084010](Plac+RBS+RhlI, Competent Cells : JW1908)(c1,c2,c4~c8)
from Glycerol Stock:
  • [http://partsregistry.org/Part:BBa_S03623 BBa_S03623](Ptet+RBS+LuxI, Competent Cells : JW1908)
--->Phenotype test
  • MIX
Sample No. 1234567891011
r3 1mL----------
r4-1mL---------
r6--1mL--------
r7---1mL-------
c1----1mL------
c2-----1mL-----
c6------1mL----
c7-------1mL---
BBa_S03154[http://partsregistry.org/Part:BBa_S03154]--------1mL--
AHL(100μM)----------1μL
BBa_T9002[http://partsregistry.org/Part:BBa_T9002]1mL1mL1mL1mL1mL1mL1mL1mL1mL1mL1mL
IPTG(100μM)1μL1μL1μL1μL1μL1μL1μL1μL---


  • Incubated for 8hours at 37 degrees
  • The measurement of the intensity GFP by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6)
Sample No. 1234567891011
the intensity GFP (Frist run) 15.2116.4416.3416.639.7209.7039.6859.87419.9516.5971.04
the intensity GFP (Second run) 17.8519.1919.2819.149.98110.1610.1410.4123.9417.6179.53


  • Spindown (max rpm, 3 min)
  • The measurement of the intensity GFP(visual judgment) (UV 312nm)
Sample No. 1234567891011
the intensity GFP ++++----+++-

Team:Output

TIME RESPONCE(liquid)

Digestion test

  • [http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2
  • [http://partsregistry.org/Part:BBa_E2030 BBa_E2030]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2
DNA(digested sample) 2
PstⅠ 0.1
BSA 0.8
Buffer3 1
dH2O 7
TOTAL 10

-->37°C 30in

Gel Check

  • [http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2①
  • [http://partsregistry.org/Part:BBa_E2030 BBa_E2030]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2②
  • [http://partsregistry.org/Part:BBa_J52008 BBa_J52008](PCR Product)③
  • [http://partsregistry.org/Part:BBa_E2050 BBa_E2050]④
Sample No. ①~②③,④
DNA(digested sample) 101
loading Dye 21
TOTAL 12μl6μl
  • Luciferase([http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]) check-->no light
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