Team:Chiba/Calendar-Home/9 September 2008

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===Team:Communication===
===Team:Communication===
-
(6/9)--->PCR of I9030
+
(6/9)--->[[Team:Chiba/protocol/PCR|PCR]] of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030]
 +
 
-
--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 
--->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
--->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
 +
{|align="justify"
 +
|[[Image:Chiba-0909-3.JPG]]
 +
|
 +
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample DNA</td>
 +
<td>60</td>
 +
</tr>
 +
<tr>
 +
<td>Loading Dye</td>
 +
<td>12</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>72μL</td>
 +
</tr>
 +
</table>
 +
::CinI
 +
|}
 +
--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
 +
::*CinI
 +
::[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
 +
::[http://partsregistry.org/Part:BBa_C0261 BBa_C0261]
 +
::[http://partsregistry.org/Part:BBa_S03154 BBa_S03154]
 +
::[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
 +
 +
 +
--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 +
{|align="justify"
 +
|[[Image:Chiba-0909.JPG]]
 +
|
 +
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample DNA</td>
 +
<td>1</td>
 +
</tr>
 +
<tr>
 +
<td>Loading Dye</td>
 +
<td>1</td>
 +
</tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O</td>
 +
<td>4</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>6μL</td>
 +
</tr>
 +
</table>
 +
:From left;
 +
::CinI -> ?
 +
::[http://partsregistry.org/Part:BBa_C0161 BBa_C0161] -> OK
 +
::[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> OK
 +
::[http://partsregistry.org/Part:BBa_S03154 BBa_S03154] -> OK (low)
 +
::Marker
 +
::[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] -> OK (low)
 +
|}
 +
 +
{|align="justify"
 +
|[[Image:Chiba-0909-2.JPG]]
 +
|
 +
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample DNA</td>
 +
<td>1</td>
 +
</tr>
 +
<tr>
 +
<td>Loading Dye</td>
 +
<td>1</td>
 +
</tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O</td>
 +
<td>4</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>6μL</td>
 +
</tr>
 +
</table>
 +
::[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] -> OK
 +
|}
 +
 +
#LuxI([http://partsregistry.org/Part:BBa_K084012 BBa_K084012])
 +
#LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
 +
#LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
 +
#Background([http://partsregistry.org/Part:BBa_R0010 R0010])
 +
#Background([http://partsregistry.org/Part:BBa_C0261 BBa_C0261])
 +
 +
:--->'''[[Team:Chiba/protocol/ligation/ligation|Ligation]]'''
 +
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample No.</td>
 +
<td>(1)</td><td>(2)</td><td>(3)</td><td>(4)</td><td>(5)</td>
 +
</tr>
 +
<tr>
 +
<td>insert-C0161</td>
 +
<td>3</td><td>-</td><td>-</td><td>-</td><td>-</td>
 +
</tr>
 +
<tr>
 +
<td>insert-C0261</td>
 +
<td>-</td><td>2</td><td>-</td><td>-</td><td>2</td>
 +
</tr>
 +
<tr>
 +
<td>insert-S03154</td>
 +
<td>-</td><td>-</td><td>6</td><td>-</td><td>-</td>
 +
</tr>
 +
<tr>
 +
<td>Vector-R0010</td>
 +
<td>-</td><td>5</td><td>5</td><td>5</td><td>-</td>
 +
</tr>
 +
<tr>
 +
<td>vector-J04500</td>
 +
<td>3</td><td>-</td><td>-</td><td>-</td><td>-</td>
 +
</tr>
 +
<tr>
 +
<td>ligase</td>
 +
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 +
</tr>
 +
<tr>
 +
<td>Buffer</td>
 +
<td>2</td><td>2</td><td>4</td><td>2</td><td>2</td>
 +
</tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O</td>
 +
<td>1</td><td>-</td><td>4</td><td>2</td><td>5</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>10</td><td>10</td><td>20</td><td>10</td><td>10</td>
 +
</tr>
 +
</table>
 +
<br clear=all>
 +
 +
:---> '''[[Team:Chiba/protocol/transformation|Transformation]]'''
 +
 +
---> '''Colony Count'''( 10/9)
 +
 +
===Team:Output===
 +
 +
no work

Latest revision as of 01:26, 30 October 2008

>Home | Notebook

8 September 2008 <|> 10 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(6/9)--->PCR of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030]


--->Gel Extract

Chiba-0909-3.JPG
Sample DNA 60
Loading Dye 12
TOTAL 72μL
CinI


--->Zymo Clean

  • CinI
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261]
[http://partsregistry.org/Part:BBa_S03154 BBa_S03154]
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]


--->Gel Check

Chiba-0909.JPG
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μL
From left;
CinI -> ?
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161] -> OK
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> OK
[http://partsregistry.org/Part:BBa_S03154 BBa_S03154] -> OK (low)
Marker
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] -> OK (low)
Chiba-0909-2.JPG
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μL
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] -> OK
  1. LuxI([http://partsregistry.org/Part:BBa_K084012 BBa_K084012])
  2. LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
  3. LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
  4. Background([http://partsregistry.org/Part:BBa_R0010 R0010])
  5. Background([http://partsregistry.org/Part:BBa_C0261 BBa_C0261])
--->Ligation
Sample No. (1)(2)(3)(4)(5)
insert-C0161 3----
insert-C0261 -2--2
insert-S03154 --6--
Vector-R0010 -555-
vector-J04500 3----
ligase 11111
Buffer 22422
dH2O 1-425
TOTAL 1010201010


---> Transformation

---> Colony Count( 10/9)

Team:Output

no work

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/9_September_2008"