Team:Chiba/Calendar-Home/9 September 2008

From 2008.igem.org

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:---> '''[[Team:Chiba/protocol/transformation|Transformation]]'''
:---> '''[[Team:Chiba/protocol/transformation|Transformation]]'''

Latest revision as of 01:26, 30 October 2008

>Home | Notebook

8 September 2008 <|> 10 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(6/9)--->PCR of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030]


--->Gel Extract

Chiba-0909-3.JPG
Sample DNA 60
Loading Dye 12
TOTAL 72μL
CinI


--->Zymo Clean

  • CinI
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261]
[http://partsregistry.org/Part:BBa_S03154 BBa_S03154]
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]


--->Gel Check

Chiba-0909.JPG
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μL
From left;
CinI -> ?
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161] -> OK
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> OK
[http://partsregistry.org/Part:BBa_S03154 BBa_S03154] -> OK (low)
Marker
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] -> OK (low)
Chiba-0909-2.JPG
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μL
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] -> OK
  1. LuxI([http://partsregistry.org/Part:BBa_K084012 BBa_K084012])
  2. LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
  3. LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
  4. Background([http://partsregistry.org/Part:BBa_R0010 R0010])
  5. Background([http://partsregistry.org/Part:BBa_C0261 BBa_C0261])
--->Ligation
Sample No. (1)(2)(3)(4)(5)
insert-C0161 3----
insert-C0261 -2--2
insert-S03154 --6--
Vector-R0010 -555-
vector-J04500 3----
ligase 11111
Buffer 22422
dH2O 1-425
TOTAL 1010201010


---> Transformation

---> Colony Count( 10/9)

Team:Output

no work

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