Team:Chiba/Sender experiments/Senders(JW1908) T9002(JW1908)

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Results and Discussion

Reaction temparature:37°C,09/12

Sender culture：1000μL,Receiver culture:1000μL

Fig.1　　
12/09.E.coli strain,senders:JW1908,BBa_T9002:JW1908,Reaction temparature:37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

Sender culture：100μL,Receiver culture：1000μL

Fig.2　　
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

Fig. 1,Fig. 2

Green fluorescence intensity didn't increase in the culture containing BBa_K0840010(plac+CinI+LVA).We thought BBa_K084010 didn't work properly or LuxR gene didn't interact with signal molecules synthesized by BBa_K084010. Others responded at the same time(4 hours after induction).Final fluorescence intensity differd.

Reaction temparature:37°C

Sender culture：500μLm,Receiver culture：500μL

Fig.3　　
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

Fig.4　　
E.coli strain,senders:JW1908,BBa_T9002:JW1908,37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

Fig. 3

Response time and final fluorescence intensity showed no significant difference. We thought AHL concentration was quickly reached the threshold concentration.The fluorescence intensity increased as gfp maturured.

Fig. 4

No significant difference in fluorescence intensity between the culture containing BBa_K084007(plac+RhlI) gene transformed cells and the culture containing BBa_K084008(plac+RhlI(LVA)) gene transformed cells.We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

Reaction temparature:30°C

Sender culture：500μL,Receiver culture：500μL

Fig.5　　
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

Fig.6　　
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

Fig. 6

No significant difference in fluorescence intensity between the culture containing BBa_K084007(plac+RhlI) gene transformed cells and the culture containing BBa_K084008(plac+RhlI(LVA)) gene transformed cells.We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

Sender culture：100μL,Receiver culture：1000μL

Fig.7　　
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

Fig.8　
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

No significant difference in fluorescence intensity between the culture containing BBa_K084007(plac+RhlI) gene transformed cells and the culture containing BBa_K084008(plac+RhlI(LVA)) gene transformed cells.We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

Sender culture：10μL,Receiver culture：1000μL

Fig.9　　
E.coli strain,senders:JW1908,BBa_T9002:JW1908,30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.

The final fluorescence intensity of the culture containing BBa_K084008 transformed cells which express LVA-tagged RhlI protein was lower than the culture containing cells which express untagged RhlI protein.It shows that LVA-tagged RhlI protein was degraded by LVA-specific protase.However,fluorescence intensity of both cultures increased at the same time.We thought it was because the AHL concentration was quickly reached the threshold concentration and cells began to express gfp.After 2 hours,fluorescence intensity started to increase.

>Back to Sender experiment and result

@@ Line 50: / Line 50: @@
 ===Reaction temparature:30°C===
 ====Sender culture：500&mu;L,Receiver culture：500&mu;L====
-[[Image:Chiba_talks_JW1908_30_RS1_01.gif|thumb|left|'''Fig.5'''　　<br>E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30&deg;C]]
+[[Image:Chiba_talks_JW1908_30_RS1_01.gif|thumb|left|'''Fig.5'''　　<br>E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30&deg;C.All measurements are averages from three replicate cultures with error bars representing standard deviations.]]
-[[Image:Chiba_talks_JW1908_30_RS1_02.gif|thumb|left|'''Fig.6'''　　<br>E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30&deg;C]]
+[[Image:Chiba_talks_JW1908_30_RS1_02.gif|thumb|left|'''Fig.6'''　　<br>E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30&deg;C.All measurements are averages from three replicate cultures with error bars representing standard deviations.]]
 <br clear=all>
@@ Line 58: / Line 58: @@
 ====Sender culture：100&mu;L,Receiver culture：1000&mu;L====
-[[Image:Chiba_talks_JW1908_30_RS2_01.gif|thumb|left|'''Fig.7'''　　<br>E.coli strain,senders:JW1908,BBa_T9002:JW1908,30&deg;C]]
+[[Image:Chiba_talks_JW1908_30_RS2_01.gif|thumb|left|'''Fig.7'''　　<br>E.coli strain,senders:JW1908,BBa_T9002:JW1908,30&deg;C.All measurements are averages from three replicate cultures with error bars representing standard deviations.]]
-[[Image:Chiba_talks_JW1908_30_RS2_02.gif|thumb|left|'''Fig.8'''　<br>E.coli strain,senders:JW1908,BBa_T9002:JW1908,30&deg;C]]
+[[Image:Chiba_talks_JW1908_30_RS2_02.gif|thumb|left|'''Fig.8'''　<br>E.coli strain,senders:JW1908,BBa_T9002:JW1908,30&deg;C.All measurements are averages from three replicate cultures with error bars representing standard deviations.]]
 <br clear=all>
 No significant difference in fluorescence intensity between the culture containing [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](plac+RhlI) gene transformed cells and the culture containing [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](plac+RhlI(LVA)) gene transformed cells.We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.
 ====Sender culture：10&mu;L,Receiver culture：1000&mu;L====
-[[Image:Chiba_talks_JW1908_30_RS3_02.gif|thumb|left|'''Fig.9'''　　<br>E.coli strain,senders:JW1908,BBa_T9002:JW1908,30&deg;C]]
+[[Image:Chiba_talks_JW1908_30_RS3_02.gif|thumb|left|'''Fig.9'''　　<br>E.coli strain,senders:JW1908,BBa_T9002:JW1908,30&deg;C.All measurements are averages from three replicate cultures with error bars representing standard deviations.]]
 <br clear=all>
 The final fluorescence intensity of the culture containing [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] transformed cells which express LVA-tagged RhlI protein was lower than the culture containing cells which express untagged RhlI protein.It shows that LVA-tagged RhlI protein was degraded by LVA-specific protase.However,fluorescence intensity of both cultures increased at the same time.We thought it was because the AHL concentration was quickly reached the threshold concentration and cells began to express gfp.After 2 hours,fluorescence intensity started to increase.